Investigation of the prevalence of Brucella Canis bacteria in aborted dogs in Shiraz

Subject Areas : Smal Animal Medicine

بهنام پدرام ¹ , حسین فتاحی ² , Behnaz Nikafraz ³ , Shaqayeq Gannad ⁴ , Hamid Khorram ⁵

1 - مربی دانشگاه آزاد اسلامی واحد سوسنگرد
2 - Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Kazerun Branch, Kazerun, Iran
3 - Doctor of Veterinary Medicine graduate, Shushtar Branch, Islamic Azad University, Shushtar, Iran
4 - Doctor of Veterinary Medicine student, Shushtar Branch, Islamic Azad University, Shushtar, Iran
5 - Ph.D in Small Animal Internal Diseases, Islamic Azad University, Science and Research Branch, Tehran, Iran

Received: 2025-04-21 Accepted : 2025-07-19 Published : 2025-07-26

Keywords: Brucella canis, ELISA, Vagina soap, Dog,

Abstract :

Brucellosis is a globally distributed zoonotic disease caused by various species of Brucella. This study aimed to investigate the presence of Brucella in serum and vaginal samples of non-neutered dogs in Shiraz. For this purpose, blood and vaginal swab samples were collected from 50 non-neutered dogs. Of these, 25 samples were taken from dog breeding centers around Shiraz and 25 from dogs referred to small animal clinics within the city. Serological analysis was performed using an IgG ELISA kit specific for Brucella canis. Among the sampled dogs, 12 (24%) were seropositive and 38 (76%) were seronegative. Of the mated female dogs, 32 (66%) were pregnant, while 17 (34%) were not. A total of 42 dogs (84%) gave birth, and 8 did not. Seven females (14%) had a history of abortion, and no abortion was observed in the remaining 43 (86%). Puppies of 7 dogs (16.28%) died, and puppies of 2 dogs (4.65%) had congenital anomalies. Vaginal swab samples were analyzed using Real-time PCR, and Brucella DNA was detected in 2 out of 50 samples (4%). According to these findings, dogs should be considered a potential reservoir of brucellosis. Detection of Brucella in vaginal samples highlights the need for more comprehensive surveillance in canine populations in this region.

References:

1. حسن آبادی،ن. رضایی،م، خلیلی،م :بررسی حضور باکتری جنس بروسلا در نمونه های سواب واژنی جمع آوری شده از سگ های ارجاعی به بیمارستان دانشکده دامپزشکی دانشگاه شهید باهنرکرمان به روش PCR.پایان نامه دانشکده دامپزشکی کرمان، دانشگاه شهید باهنر کرمان،سال تحصیلی1397.
2. میرشکاری،پ. سرچاهی،ع ا، مهری،م : بررسی مقدماتی سرولوژیکی بروسلا کنیس در شهرستان مشهد.پایان نامه دانشکده دامپزشکی مشهد، دانشگاه فردوسی مشهد ،سال تحصیلی1398.
3. Dahlbom, M.; Johnsson, M.; Myllys, V.; Taponen, J. and Andersson, M. (2009). Seroprevalence of canine herpesvirus-1 and Brucella canis in Finnish breeding kennels with and without reproductive problems. Reproduction Domestic Animals, 44 (1): 128-131.
4. شیمی، احمد. باکتری شناسی دامپزشکی و بیماری های باکتریایی, باکتری شناسی دامپزشکی و بیماری های باکتریایی، چاپ اول، موسسه نشر جهاد دانشگاهی, 1376 , صفحات 337 - 307 .
5. مشهدی رفیعی، سیامک؛ آل داود، سید جاوید؛ نجار، محمدهادی. بررسی سرو اپیدمیو،وژی بروسلوز در سگ های بومی شهرستان نیشابور, مجله دانشکده دامپزشکی دانشگاه آزاد واحد گرمسار, 1389 , صفحات 6 - 1
6. احمدی، ملاح ؛ دستمالچی ساعی، حبیب. اصول باکتری شناسی دامپزشکی, اصول باکتری شناسی دامپزشکی ، تالیف جی. آر، کارتر و دالارجی، وایس. چاپ اول، انتشارات جهاد دانشگاهی ارومیه, 1387 , صفحات 262 - 247 .
7. Dahlbom, M.; Johnsson, M.; Myllys, V.; Taponen, J. and Andersson, M. (2009). Seroprevalence of canine herpesvirus-1 and Brucella canis in Finnish breeding kennels with and without reproductive problems. Reproduction Domestic Animals, 44 (1): 128-131.
8. Christopher S, Umapathy B, Ravikumar K. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. Journal of laboratory physicians. 2010;2(2):55
9. Megid J, Mathias LA, Robles C. Clinical manifestations of brucellosis in domestic animals and humans. The Open Veterinary Science Journal. 2010:119-26.
10. Cosford KL. Brucella canis: An update on research and clinical management. The Canadian Veterinary Journal. 2018;59(1):74.
11. ذوقی، اسماعیل.“ سیمای بالینی و آزمایشگاهی بروسلوز در انسان تا لیف ادوارد جی یانگ، میخائیل جی کوربل.چاپ اول، موسسه انتشاراتی سازمان نظام دامپزشکی, 1382 , صفحات 156 - 94
12. Adesiyun AA, Abdullahi SU, Adeyanju JB. Prevalence of Brucella abortus and Brucella canis antibodies in dogs in Nigeria. J Small Anim Pract. 1986 Jan;27(1):31–7.
13. Capasso L. Bacteria in two-millennia-old cheese, and related epizoonoses in Roman populations. Journal of Infection. 2002;45(2):122-7.
14. قربانپور نجف آبادی،م. مصلی نژاد،ب، محمدیان،ن :جستجوی آنتی بادی ضد بروسلوز(بروسلا کنیس) در سگ های شهری و روستایی شهرستان اهواز به روش ایمنوکروماتوگرافی.پایان نامه دانشکده دامپزشکی اهواز، دانشگاه شهید چمران اهواز،سال تحصیلی1389.
15. De Figueiredo P, Ficht TA, Rice-Ficht A, Rossetti CA, Adams LG. Pathogenesis and immunobiology of brucellosis: review of Brucella–Host Interactions. The American journal of pathology. 2015;185(6):1505-17.
16. Ruiz-Mesa J, Sanchez-Gonzalez J, Reguera J, Martin L, Lopez-Palmero S, Colmenero J. Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas. Clinical microbiology and infection. 2005;11(3):221-5.
17. Smits HL, Kadri SM. Brucellosis in India: a deceptive infectious disease. Indian Journal of Medical Research. 2005;122(5):375.
18. Keid LB, Soares RM, Vasconcellos SA, Chiebao D, Salgado V, Megid J, et al. A polymerase chain reaction for detection of Brucella canis in vaginal swabs of naturally infected bitches. Theriogenology. 2007;68(9):1260-70.56.
19. Franco MP, Mulder M, Gilman RH, Smits HL. Human brucellosis. The Lancet infectious diseases. 2007;7(12):775-86.
20. Dentinger CM, Jacob K, Lee LV, Mendez HA, Chotikanatis K, McDonough PL, et al. Human Brucella canis infection and subsequent laboratory exposures associated with a puppy, New York City, 2012. Zoonoses and public health. 2015;62(5):407-14.
21. Brown J, Blue J, Wooley R, Dreesen D, Carmichael L. A serologic survey of a population of Georgia dogs for Brucella canis and an evaluation of the slide agglutination test. Journal of the American Veterinary Medical Association. 1976;169(11):1214-6.
22. Higgins R, Hoquet F, Bourque R, Gosselin Y. A serological survey for Brucella canis in dogs in the province of Quebec. The Canadian Veterinary Journal. 1979;20(11):315.