Prevalence of class 1 and 2 integrons in uropathogenic Escherishia coli isolates from diabetic patients in Kermanshah, Iran

Subject Areas : microbiology

Rasul Pajohesh ¹ , fatemeh Khodaverdipour ² , Hossein Aghajani dezaki ³

1 - 1. Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran
2 - 1. Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran
3 - 1. Department of Microbiology, ShK.C., Islamic Azad University, ShahreKord, Iran

Received: 2025-07-11 Accepted : 2025-07-12 Published : 2025-07-11

Keywords: Escherishia coli, Integrons, Multi Drug Resistance, Urinary Tract Infection,

Abstract :

Escherichia coli is one of the most predominant pathogens causing 80-90% of community acquired UTIs and 30-50% of nosocomial acquired UTIs. Multi drug resistance isolates encoded by linked resistance genes occurs on integrons.

In this cross sectional study, a total number of 90 E. coli isolates were obtained from urine samples of diabetic patients in Kermanshah, Iran, during a six-month period (October to March, 2015). Urine samples were tested by standard bacteriological methods and antibiotic susceptibility tests were done for all isolates by Kirby Bauer agar disk diffusion. Detection of class 1 and class 2 integrons were performed by PCR in MDR isolates.

The most common resistant phenotypes were found to be Cefalothin (88.88%), Nalidixic acid (83.33%), Co-trimoxazole (70%), Ciprofloxacin (58.88%), tetracycline (56.66%), Norfloxacin (55.55%), Ceftazidim (40%), Gentamycine(32.23%), Choloramphenicol (26.66%), Nitrofurantion (21.11%), Amikacin (17.77%) and Imipenem (2.22%). In this study, a total of 90 isolates in 76 samples were reported MDR. Class I integron was found in16 isolates (21.05%) and class 2 integron was found in 8 isolates (10.52%). No strain was found to contain both class 1 and 2 integrons. In this study,

There was a significant relationship between the presence of the intI1 gene and resistance to Norfloxacin and Cephalotin. This indicated that the antibiotic resistance genes were located within the integron

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