A preliminary study of the effects of the different doses of testosterone and tacrolimus on gasterocnemius muscle atrophy caused by experimental sciatic nerve damage in rats

Subject Areas :

M Jazini ¹ , H.R. Fattahian ² , Abbas Aliaghaei ³ , MA Abdolahifar ⁴

1 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Scienceand Research Branch, Islamic Azad University, Tehran-Iran.
3 - Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - 1Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Received: 2025-01-15 Accepted : 2025-03-18 Published : 2025-03-19

Keywords: sciatic nerve, testosterone, tacrolimus, gastrocnemiu,

Abstract :

Treatment of muscle atrophy, even after the regeneration of the damaged nerve, is one of the major problems of clinicians. Therefore, the purpose of this research is to investigate different amounts of two drugs in healing the damaged nerve and preventing atrophy caused by not using muscles after sciatic nerve damage. In this study, 20 rats were divided into four groups of five. After anesthetizing and exposing the sciatic nerve, this nerve was crushed with forceps for 10 seconds. Then the rats were placed in the groups testosterone one (group one), testosterone two (group two), tacrolimus one (group three) and tacrolimus two (group four) and respectively testosterone (subcutaneous, 5 mg/kg), testosterone (subcutaneous, 10 mg/kg), tacrolimus (gavage, 5 mg/kg) and tacrolimus (gavage, 10 mg/kg) were recieved during 28 days. On the 28th day, the rats were humanely killed and morphometric and histological evaluations were performed. In evaluating the wet weight of the gastrocnemius muscle, a statistically significant difference was observed in groups 1 and 2 (P>0.05), while this evaluation did not show a significant difference between groups 3 and 4 (P>0.05). The researchers of the current study believe that according to the information and findings, they suggest the consumption of 5 mg/kg of testosterone and 5 mg/kg of tacrolimus in restoring nerve function and reducing atrophy after experimental nerve damage.

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