Changes in the expression of the tumor suppressor gene (TP53) in liver tissue and oxidative markers following six weeks of resistance training and pineapple extract consumption in mice with melanoma cancer
Subject Areas : Molecular detection of biochemical and genetic markers
saeid Shahrestani
1
,
حسین عابدنطنزی
2
,
Bahram Abedi
3
,
شهرام سهیلی
4
1 - 1- Ph.D. Student of Exercise physiology, Science and research branch, Islamic Azad University, Faculty of Humanities, Department physical education and sport science, Tehran, Iran.
2 - Department physical education and sport science, Science and research branch, Islamic Azad University,Tehran,Iran
3 - Department of Sports Sciences, Islamic Azad University, Mahalat Branch, Mahalat, Iran
4 - دانشگاه آزاد اسلامی واحد شهرقدس. گروه تربیت بدنی
Keywords: resistance training, pineapple, melanoma cancer, tumor, P53,
Abstract :
Background & Aim: Melanoma cancer is the most severe subtype of skin cancer, which has high invasiveness and rapid metastasis to other organs. Recently, the role of sports training in the prevention and treatment of cancer has received much attention. Pineapple also belongs to the bromolyase family and the bromoloid subfamily, whose anti-cancer properties have been suggested. P53 gene is a tumor suppressor gene. The aim of the present study was to study the effect of six weeks of resistance training and consumption of pineapple extract on tumor volume and tumor tissue P53 gene expression in mice with melanoma cancer.
Materials & Methods: The basic study began with 32 C57 mice as a sample, which was eventually divided into four groups, including the remaining 20 mice in four groups including control, resistance training, pineapple extract and resistance training-pineapple. After tumor induction in mice, the program of resistance training and pineapple extract at the rate of 300 mg/kg was implemented for six weeks. The weight and tumor volume of mice were measured. Measurement of P53 gene expression in tumor tissue was done by RT-PCR method. Then the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc test. A significance level of p≤0.05 was considered.
Results: Results was shown that compared to the control group, resistance training and pineapple-based training significantly reduced tumor volume and decreased P53 gene expression in tumor tissue and increased GPX antioxidant index and decreased MDA oxidative stress index in experimental groups, while in the interaction group, the mean changes and effectiveness were greater.
Conclusion: Resistance training and pineapple extract led to weight loss and significant reduction in tumor volume and increased expression of the tumor suppressor p53 gene and increased GPX antioxidant index and decreased MDA oxidative stress index in experimental groups compared to the control group in tumor tissue.
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