Investigating the effect of Astragalus hamosus extract on hepatotoxicity caused by thioacetamide in rats
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)Ghazaleh maleki 1 , Elham Moghtadaei Khorasgani 2
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Keywords: Liver, Astragalus hamosus, thioacetamide (TAA), antioxidant,
Abstract :
Background & Aim: Thioacetamide (TAA) is a potent hepatotoxin that destroys liver cells. The use of plants is increasing day by day due to their protective effect against diseases such as cancer. The purpose of this study was to investigate the effect of the Astragalus hamosus extract on liver toxicity induced by TAA. Experimental: 32 rats were randomly divided into four groups of eight. The first group only received food and water rations. The second group received only TAA (50 mg/kg). The third group received both TAA and A. hamosus extract at a dose of 200 mg/kg. The fourth group received the extract at a dose of 400 mg/kg along with TAA. To investigate and compare liver function in different groups, serum transaminases including alanine aminotransferase alanine transaminase (ALT), aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT), gamma-glutamyl transferase (GGT), and malondialdehyde (MDA) were measured. Then the samples were examined histopathologically. Results: The best effect was observed with a dose of 400 mg of A. hamosus extract which significantly reduced SGPT and SGOT levels (P<0.05). Additionally, the extract at a dose of 400 mg significantly reduced ALP, MDA levels, which were increased due to TAA consumption (P<0.05). Histopathological analysis of liver tissue in the groups showed that liver cells in the TAA group exhibited central venous hyperemia and necrosis. However, liver cells were healthy in the group receiving the extract at a dose of 400 mg. This study showed that the effect of the extract was dose-dependent, and the most significant effect was observed with a dose of 400 mg of the extract. Recommended applications/industries: The present findings showed that the A. hamosus extract, probably due to its antioxidant properties, will modulate liver enzymes and improve the tissue structure of the liver in hepatotoxicity.
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