The Effects of eight weeks of resistance, endurance, and combined training on Mir-208a and Mir-208b in inactive young girls
Subject Areas : Cellular & Molecular Exercise Biology and Sports Genetic
Shahrzad Ansari
1
,
Tahereh bagherpoor
2
,
Nematollah Nemati
3
1 - Department of Sports Sciences, Da. C., Islamic Azad University, Damghan, Semnan, Iran.
2 - Department of Sports Sciences, Da. C., Islamic Azad University, Damghan, Semnan, Iran.
3 - Department of Sports Sciences, Da. C., Islamic Azad University, Damghan, Semnan, Iran.
Keywords: Exercise Training, Mir-208a, Mir-208b, Muscle Adaptation, Molecular Genetics,
Abstract :
Skeletal muscles play a fundamental role in key body functions. Therefore, maintaining skeletal muscle health is important. Prolonged periods of muscle inactivity lead to skeletal muscle atrophy and weakness. Performing daily activities requires adequate muscle size and strength, and atrophy has a negative impact on overall quality of life; as decreased skeletal muscle mass leads to reduced performance, health, and quality of life.
The present quasi-experimental study aimed to determine and compare the effects of eight weeks of resistance, endurance, and combined training on Mir-208a and Mir-208b in inactive young girls with a three-group research design without a control group in the pre-test and post-test. Resistance training with weights was performed for eight weeks, three to four sessions per week, in the intensity range of 80-95% of one repetition maximum, observing the principle of progressive overload, with three to five sets per movement, in a circular manner, for approximately 90 minutes per session. Endurance training was performed in the form of running and walking on a treadmill with a 0% incline and a speed of 3.5-9 km/h for 25-45 minutes and pedaling on a stationary bike with a power of 100-150 watts for 10-35 minutes in a circular manner for eight weeks, three to four sessions per week, observing the principle of increasing overload, for approximately 90 minutes per session. The relative expression levels of MyomiRs were determined using RT-qPCR and RT Stem-Loop techniques. The MyomiR measurement kit with the trade name BiomiR (MicroRNA Expression Measurement Kit - MI001) produced by Anacell Company was used, which is capable of determining the expression level of MyomiRs in RNA extracted from serum with very high specificity and sensitivity (up to less than 100 copies of MicroRNA). Statistical analysis of data was performed with one-way analysis of variance (P≤.05).
The mean difference in relative expression of Mir-208a gene in the endurance group was 0.67 ± 0.12, in the resistance group was 0.55 ± 0.12, and in the combination group was 0.35 ± 0.12, which was significant. Also, the mean relative expression of Mir-208b gene was 0.58 ± 0.10 in the endurance group, 0.50 ± 0.08 in the resistance group, and 0.34 ± 0.09 in the combination group, which was significant in all groups. Based on the findings of the present study, it can be concluded that resistance, endurance, and combined exercises have an effect on Mir-208a and Mir-208b in inactive young girls. The effect of exercise on epigenetic changes appears to depend on the type, intensity, and duration of exercise. In this context, resistance and endurance exercise differ in their transcriptional regulation in skeletal muscle.
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