Comparison effects of silver, copper, and zinc oxide nanoparticles on the tumor necrosis factor-α (TNF-α) gene expression in infected Balb/C mice with Escherichia coli
Subject Areas : Molecular MicrobiologyMaryam Mohammadkhani 1 , جواد آراسته 2 , saba taheri 3
1 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 - دانشکده علوم پایه، گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد تهران مرکزی
3 - Department of biology, faculty of science. university of islamshahr, Islamshahr, Iran
Keywords: : Escherichia coli, Nanoparticles, Silver, Zinc oxide, Copper, Tumor necrosis factor-alpha,
Abstract :
Background & Objectives: The number of people infected by pathogenic bacteria is increasing. Routine antibiotics lose their effectiveness due to the strategies of these bacteria in resistant to antibiotics. Nanotechnology has introduced an approach called nanoparticle-based antimicrobial agents to inhibit bacteria growth. Materials & Method: Minimum inhibitory concentrations (MICs) of silver (Ag), zinc oxide (ZnO), and copper (Cu) NPs against Escherichia coli ATCC 25922 were determined using microbroth dilution. Balb/C male mice (n=54) were divided into nine groups, infected intraperitoneally with E. coli (10^8 CFU/mL), and treated with Ag, ZnO, or Cu NPs (MIC doses) or imipenem (control). Spleen tissue RNA was extracted 24 h post-treatment, and tumor necrosis factor-α (TNF-α) gene expression was quantified via Real-time PCR. Results: MICs were 125 µg/mL (Ag) and 62.5 µg/mL (ZnO, Cu), indicating superior efficacy of ZnO and Cu NPs. TNF-α expression was significantly lower in imipenem and Ag NP groups compared to ZnO and Cu NP groups (p < 0.05). Conclusion: Regardless of the antibiotics, NPs also exhibit an antimicrobial property against bacteria like E. coli. These characteristics make them the proper candidate for the treatment of infectious diseases.
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