The role of antioxidant activity of nano-chitosan-hydroalcoholic extract of Bee pollen in combating aluminum chloride-induced toxicity in rat brain tissue
Subject Areas : biochemistry
1 - Assistant Prof , Department of Biology, CT.C., Islamic Azad University, Tehran, Iran
Keywords: Bee pollen, aluminum chloride, brain tissue, oxidative stress indices, rat.,
Abstract :
Introduction: Aluminum is a metal with known toxicity to humans and animals that plays a role in the pathogenesis of various diseases. Plants contain a wide range of antioxidants that can neutralize free radicals and thus prevent the progression of many chronic diseases associated with oxidative stress. The aim of this study was to investigate the effects of nanochitosan carrying a hydroalcoholic extract of flower pollen on the antioxidant level of brain tissue in male rats exposed to aluminum chloride.
Materials and Methods: In this experimental study, 28 adult male Wistar rats were divided into 4 groups of 7: control, receiving aluminum chloride (100 mg/kg), nanochitosan (1 ml), aluminum chloride + nanochitosan carrying a hydroalcoholic extract of Bee pollen (200 mg/kg). Contamination induction and nanochitosan intake were performed by gavage. After the end of the treatment period and dissection of the animals, brain tissue was extracted to measure some oxidative stress indices in different groups. Data analysis in different groups was performed with SPSS software and one-way variance statistical test, and P<0.05 was considered significant.
Results: The results obtained from this study showed that in the group receiving aluminum chloride, an increase in malondialdehyde and a decrease in superoxide dismutase and glutathione peroxidase were significantly shown compared to the control group (P<0.001), while in the group treated with nanochitosan carrying the hydroalcoholic extract of Bee pollen grains, a decrease in malondialdehyde and an increase in superoxide dismutase and glutathione peroxidase were shown compared to the infected group (P<0.05).
Conclusion: According to the findings of this study, nanochitosan carrying the hydroalcoholic extract of Bee pollen grains can probably be used as a pharmaceutical product for the prevention or treatment of damage caused by pollutants such as aluminum chloride.
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