The effectiveness of Micrococcus luteus bacteria on some blood and immune indices of common carp fry

Subject Areas : Journal of Animal Biology

Mehran Avakh Keysami ¹ , Alireza Akbari ² , Hamid Abdollahpour Biria ³ , Maryam Avakh Keysami ⁴

1 - . Department of Fisheries and Aquatics, Mirzakochek Khan Fisheries Sciences and Industries Training Unit, Agricultural Research, Education and Extension Organization Rasht, Iran.
2 - . Department of Fisheries and Aquatics, Mirzakochek Khan Fisheries Sciences and Industries Training Unit, Agricultural Research, Education and Extension Organization Rasht, Iran.
3 - Department of Fisheries, Tal.C, Islamic Azad University, Talash, Iran
4 - Research Department of Experimental Sciences Education, General Directorate of Education, Gilan Province, Bint Al-Hoda Sadr Educational Campus, Rasht, Iran

Received: 2025-05-11 Accepted : 2025-06-12 Published : 2025-07-02

Keywords: Common carp, Micrococcus luteus, Blood indices, Immune indices,

Abstract :

This research was conducted to investigate the effect of Micrococcus luteus bacteria on the blood indices, and immunology of common carp fry (Cyprinus carpio) in Mirzakochek Khan Training Center and Shahid Ansari Bony Fish  Center in Gilan was carried out in 2023.  Micrococcus luteus isolated from the digestive tract of common carp was fed to commercial pellet feed of 300 pieces of common carp juveniles (28.018 ± 2.87) for 8 weeks with 5 treatments and 3 replications (20 pieces of fish in each tank) used. Mixing fish feed in the suspension of Micrococcus luteus, 5 types of diets with different concentrations of Micrococcus in the pellet including 10⁶, 10⁷ cells/g were prepared as treatments 1, 2 and control without bacteria. After 56 days of feeding with these treatments, different levels of Micrococcus luteus bacteria in the diet of common carp fry increase the amount of The values ​​of hematocrit, red blood cells, and hemoglobin, values ​​of MCV and MCH MCHC, neutrophil and monocyte, sodium, lysozyme, and C3 in treatments 1 and 2 were significantly higher than the control treatment. In this regard, treatment 3 had the highest values, but the amounts of calcium, phosphorus, IGM, and C4 in the control treatments, treatments 1 and 2 did not have a statistically significant difference. There was a significant difference between the number of gram-positive and gram-negative bacteria in the digestive tract and water of the rearing tanks compared to the control treatment (P<0.05). Analysis of the results showed the benefits of using M. luteus probiotic in the diet of common carp fry in some cases. M. luteus is usually not pathogenic for fish. Therefore, the use of this probiotic in common carp farming to increase the ability deal with diseases is recommended.

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