Prevalence of Giardia Duodenalis Parasite in Domestic Dogs of Dezful Region by PCR Method
Subject Areas : Smal Animal MedicineMorteza Razaghi manesh 1 , Leila Derakhshan 2 , Negar Safikhani 3 , Hamidreza Pishbin 4
1 - Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University,Shushtar, Iran
2 - Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University,Shushtar, Iran
3 - Doctor of Veterinary Medicine Student, Faculty of Veterinary Medicine, Islamic Azad University, Shushtar Branch, Shushtar, Iran
4 - Doctor of Veterinary Medicine Student, Islamic Azad University, Shushtar Branch, Iran
Keywords: Giardia Duodenalis, Dog, Dezful, PCR-RFLP,
Abstract :
Despite reports of Giardia duodenalis infection in dogs in Iran, few studies have been conducted on determining the genotypes of Giardia duodenalis. The purpose of this study was to determine the prevalence of infection in domestic dogs in the Dezful region and to determine the genotypes of Giardia duodenalis in domestic dogs in Dezful. PCR-RFLP method was used for the presence of Giardia duodenalis parasite and also to determine its genotypes. In this method, the target fragment of 432 base pairs was amplified. Finally, in microscopic examination, 7 samples were infected with Giardia duodenalis parasite. Therefore, the prevalence of parasites was (2.84%). The samples using NLAIV enzyme showed that 3 samples (42/85) of genotype C, 2 samples belonging to subgroup D (28/57) and 2 samples belonging to subgroup D respectively. AI is (28/57). Our findings indicate that Giardia duodenalis infection is common in Dezful dogs. Based on these findings, it is concluded that Giardia duodenalis genotype C and D are the most common genotypes in the study area. But contamination with zoonosis genotype A is also seen. Therefore, dogs can be introduced as a potential source of human giardia through direct contact or through surface water contamination
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