Determining some characteristics of the amino acid sequences of the enhancin protein in baculoviruses
Subject Areas : Virologyمریم راشکی 1 , Mojtaba Mortazavi 2 , Ma. Cristina Del Rincón-Castro 3
1 - Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2 - Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
3 - Food Department and Postgraduate of Biosciences, Life Sciences Division, University of Guanajuato, Irapuato, Gto, México.
Keywords: Amino acid sequence, Baculoviridae, enhancin, physicochemical parameters.,
Abstract :
Background & Objectives: Among all known entomopathogenic viruses, the most extensively studied ones are related to Baculoviridae. One protein found in baculoviruses is called enhancin. This protein facilitates the breakdown of proteins, particularly mucin, in the peritrophic membrane of insects and enhances the permeability of baculoviruses. Materials & Methods: Based on the phylogenetic tree of the 67 amino acid sequences of the enhancin enzyme, nine sequences were selected. The physicochemical characteristics of the sequences were studied using the ProtParam Expasy server. Features such as stability, isoelectric point, Gravy, aliphatic chains, and light absorption coefficient were studied. Results: In this research, the instability index of the selected proteins was less than 40, a predictor of protein stability. An isoelectric point greater than seven proves the alkaline nature of the protein. In this study, the isoelectric points of the selected proteins were found to be below seven. The minimum and maximum GRAVY values were observed in the amino acid sequences of HaGV. The aliphatic index is associated with the thermal stability of proteins, with the aliphatic index range for the selected proteins being between 82.67 and 100.77. The light absorption coefficient indicates the amount of light a protein absorbs at a specific wavelength. Conclusion: This measurement estimates purification time by monitoring protein with a spectrophotometer. Considering the role of enhancins in boosting the effectiveness of baculoviruses as biological control agents, predicting the three-dimensional structures of all nine enhancin proteins is essential for identifying those that may exhibit the highest activity against insects.
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