Comparison of the Therapeutic Effects of Nano-Essence of Artemisia Plant with Terbinafine 1% Cream in the Treatment of Guinea Pigs Infected with Dermatophytosis Caused by Trichophyton mentagrophytes
Subject Areas : Smal Animal Medicine
morteza razaghimanesh
1
,
Siamak Mashhadi Rafiei
2
,
Farnosh Arfaei
3
,
Erfan Javidi
4
,
Hamid Reza Shabani
5
,
Ava Abrisham Kar
6
1 - Department of Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University,Shushtar, Iran
2 - Department of Veterinary Medicine, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
3 - Department of Veterinary Medicine, Faculty of Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
4 - Doctor of Veterinary Medicine Student, Islamic Azad University, Shushtar Branch, Iran
5 - Doctor of Veterinary Medicine Student, Islamic Azad University, Shushtar Branch, Iran
6 - Animal Biology student at the University of Guelph, Ontario, Canada
Keywords: Nano-essence of Artemisia, Terbinafine, Trichophyton mentagrophytes, Guinea pig,
Abstract :
Transforming pharmaceutical compounds into nano-sized particles grants them novel physicochemical and therapeutic properties. In this study, the nano-essence of the medicinal plant Artemisia was evaluated for its antifungal effects against experimental dermatophytosis caused by Trichophyton mentagrophytes in guinea pigs. Both in vivo and in vitro methods were employed. The minimum inhibitory concentration (MIC) of the nano-essence was determined using the Broth Microdilution method according to the Clinical Laboratory Standards Institute (CLSI) M38-A guidelines and was found to be 0.5 µg/ml.
Twenty-four guinea pigs were randomly divided into four groups of six. Treatment began five days after infection and was administered every 12 hours for 40 days. Complete clinical recovery was observed in both groups treated with the nano-essence and topical terbinafine cream by the end of the study. These findings indicate that the Artemisia nano-essence is effective in treating dermatophytosis caused by T. mentagrophytes and may even demonstrate superior therapeutic performance compared to conventional topical antifungal treatments.
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