Investigation of the Antibiotic Resistance Pattern in Klebsiella Pneumoniae Strains Isolated from Clinical Cases in Isfahan City

Subject Areas : microbiology

Hossein Khodabandeh shahraki ¹ , sara bagheriKakash ²

1 -
2 - دانشگاه ازاد شهرکرد

Received: 2025-04-15 Accepted : 2025-04-17 Published : 2025-02-11

Keywords: Antibiotic resistance, Klebsiella pneumoniae, Resistance genes,

Abstract :

Klebsiella drug resistance is increasing day by day and therefore, performing drug resistance tests before prescribing antibiotics seems essential. The aim of this study is to determine the antibiotic resistance pattern in Klebsiella pneumoniae strains isolated from clinical cases in Isfahan city. The present study was conducted on 90 Klebsiella pneumoniae isolates isolated from clinical samples from patients referred to medical diagnostic laboratories in Isfahan city. Using biochemical methods and molecular techniques based on 16srRNA gene detection, all strains were confirmed and the antibiotic resistance of the isolates was investigated using the disk diffusion method and molecular methods based on (qnr, tet A, tet B, aac (3)IIa and sul1) genes. Of the 90 strains studied, the antimicrobial resistance pattern was estimated to be the highest microbial resistance to penicillin (93.75%) and the highest sensitivity to vancomycin (100%). The aac(3)IIa gene (encoding gentamicin resistance) with a frequency of 83.33% and the qnr gene (encoding ciprofloxacin resistance) with a frequency of 16.16% were the most and least frequently detected antibiotic resistance genes in Klebsiella pneumoniae strains. Multidrug-resistant Klebsiella pneumoniae is a serious risk for patients visiting the hospital, so monitoring antibiotic use and identifying drug-resistant strains can prevent the development of resistance in bacteria.

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