Exploring Novel Phytase-Producing Escherichia coli: Isolation, Characterization, and Industrial Potential
Subject Areas : Biotechnological Journal of Environmental MicrobiologyShabnam Golbouy Daghdari 1 , Ziba Hemmati 2
1 -
2 - 1 Department of Science and Food Industries, College of Agriculture Afagh Higher Education Institute, Urmia, Iran
Keywords: Escherichia coli, Phytase, Optimization, Characterization,
Abstract :
Bacterial phytases catalyze the hydrolysis of phytic acid into myo-inositol and phosphoric acid, presenting versatile applications in environmental protection, agriculture, and animal feed industries. This study isolates and assesses the stability of phytase-producing Escherichia coli from soil, emphasizing their potential for commercial utilization.Top of Form
Samples were obtained from soil contaminated with animal and poultry feces. Phytase-producing bacteria were isolated on PSM media, and identification was based on biochemical properties, morphological characteristics, and molecular analysis, specifically the 16S rDNA gene.
The optimal time for phytase activity was determined by spectrophotometric measurement of liberated phosphorus in the liquid medium. Various pH levels and temperatures were tested to evaluate enzyme activity and stability. The 16S rDNA gene sequences of the isolates (ZH2 and ZH3) exhibited 99% and 100% homology with E. coli. Maximum phytase production occurred between 16-24 hours after incubation. Results indicated that bacterial isolates (ZH2 and ZH3) displayed enzyme activity at pH 3 and 9, and these newly identified strains produced significant amounts of the enzyme at 37 °C.
The study provides comprehensive insights into the potential applications of these newly isolated strains, suggesting their promising role in phytase enzyme production. The adaptability of these strains to diverse pH conditions and their activity at 37°C positions them as valuable candidates for applications in animal feed supplementation and environmental management.
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