Improving the Cryostorage Parameters of Ram Germ Stem Cells by Supplementing with Soybean Lecithin as a Cryoprotectant
Subject Areas :S. Ghahremani 1 , M. Sharafi 2 , B. Ghasemi Panahi 3 , A. Javanmard 4
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Keywords: cryopreservation, cryoprotectants, Ghezel sheep, soybean lecithin, sper-matogonial stem cells,
Abstract :
To date, the challenging problem of reducing cryodamage caused by the freezing and thawing process of spermatogonial stem cells (SSCs) is still at the experimental stage, and understanding the complexity of this puzzle is crucial. More recently, soybean lecithin (SL) has been claimed to be a suitable molecule to protect SSCs from cold shock throughout the cryostorage process. Based on this motivation, the main aim of the present research is to evaluate the cryostorage parameters of ram germinal stem cells by SL supplementa-tion as a cryoprotectant agent. For this purpose, SSCs were isolated from the testicular tissue of a 2-month-old ram and we then added different concentrations of SL (control, 0.5, 1 and 1.5 percent) and then added them to the basic media described. Next, cryostorage parameters (cell viability, number and area of colo-nies) were assessed per treatment. To examine this statistically, we calculated a one-way ANOVA and HSD test with SAS and a P < 0.05 was considered significant. Interestingly, our results showed that cell viability and colony number and area were significantly higher during supplementation with 0.5% SL than the other treatments (P<0.05). However, SSCs preserved in 1.5% SL have adverse effects due to their toxicity rate. On this basis, our current findings demonstrate an improvement in cryostorage parameters of ram germ cells by supplementation with SL. This assumption could be addressed in future studies.
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