Influence of Different Amounts of Molasses and Microbiologically Treated Potato Aerial Part on in vitro Gas Production

Subject Areas :

S. Najafyar ¹ , H. Paya ² , A. Taghizadeh ³ , A. Hosseinkhani ⁴ , H. Mohammadzadeh ⁵

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Received: 2024-03-14 Accepted : 2024-11-15 Published : 2024-12-01

Keywords: alfalfa hay, in vitro gas production, microbial additive, molasses, processed po-tato aerial part,

Abstract :

This project aimed to study the effects of in vitro gas production when varying amounts of molasses- and microbial additive-treated aerial part of the potato are added in place of alfalfa hay in a total mixed ration (TMR). The experiment used three treatments and three replicates in a completely randomized design. 1) control (100% alfalfa hay in the fodder part of the diet), 2) T50 (50% alfalfa hay, 50% processed aerial part of potato in the fodder part of the diet) and 3) T100 (100% processed aerial part of potato in the fodder part of the diet) were the treatments. To collect rumen fluid, two fistulated male sheep were used. After incuba-tion, the in vitro gas production amount was measured at 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 hours. After two hours of incubation, no difference was apparent between the experimental treatments. Treatments T50 and T100 showed no significant difference in the 4th to 12th hour of incubation, but both showed a signifi-cant difference from treatment control (P≤0.05). Furthermore, all three treatments (control, T50 and T100) showed a highly significant difference (P≤0.001) in in vitro gas production from the 16th to the 96th hour of incubation. Treatments control, T50, and T100 produced 52.67, 61.6, and 68.53 ml of in vitro gas per 300 mg dry matter, respectively, based on the total volume of in vitro gas production. On this basis, lambs fed TMR instead of alfalfa hay can to utilize all of the processed aerial part of potato.

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