High Intensity Interval Training Improves GLUT4 Gene Expression in the Soleus Muscle of Male Diabetic Rats

Subject Areas : Physical Activity and Health

Esrafil Faraji ¹ , Kamal Azizbeigi ² , Khalid Mohammad Zadeh Salamat ³ , ظاهر اعتماد ⁴

1 - Department of physical education, Sanandaj branch, Islamic Azad University, Sanandaj, Iran
2 - Department of physical education, Sanandaj branch, Islamic Azad University, Sanandaj, Iran
3 - Assistant Professor, Department of Physical Education, Sanandaj Branch, Azad University Eslami, Sanandaj, Iran
4 - گروه تربیت بدنی ،دانشگاه آزاد اسلامی

Received: 2024-12-06 Accepted : 2025-01-31 Published : 2025-07-26

Keywords: Glucose Metabolism, High-intensity exercise, Insulin Resistance. ,

Abstract :

Aim: Increasing insulin receptor gene expression can be effective in controlling blood sugar. The aim of the present study was to investigate the effect of high-intensity interval training (HIIT) on changes in GLUT4 gene expression in the soleus muscle in diabetic male rats.

 Method: 16 Wistar rats were selected and randomly assigned to diabetic control (Con; n=8) and high-intensity interval training (HIIT; n=8) groups. After two weeks of intraperitoneal injection of low-dose streptozotocin (STZ) and after ensuring that the rats become diabetic, HIIT was performed five sessions per week for six weeks in 8-10 sets at an intensity of 65-85% of maximum oxygen consumption (VO2max) on a treadmill. After killing the animals and preparing the soleus muscle tissue, GLUT4 gene expression was measured by real-time PCR.

Results: It was observed that the distribution of GLUT4 variable data in the studied groups had a normal distribution (p=0.268, p=0.429 respectively for Con and HIIT). It was observed that there was a significant difference in GLUT4 gene expression changes between the HIIT and Control (p=0.001), such that its expression increased significantly in the HIIT (p=0.001).

Conclusion: Finally, it can be said that intense interval training will increase GLUT4 gene expression in the soleus muscle of diabetic rats

References:


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