The Effect of High-Intensity Interval Training and High-Protein Diet on Plasma Levels of Apolipoprotein A1 and B and Lipid Profile in obese Male Rats

Subject Areas : Journal of Animal Biology

Marziyeh Saghebjoo ¹ , Mahdi Aliakbari ² , Haniyeh Habibi Sangani ³

1 - Department of Sport Sciences, Faculty of Sport Sciences, University of Birjand, Birjand, Iran
2 - Department of Physical Education and Sport Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Sport Sciences, Faculty of Sport Sciences, University of Birjand, Birjand, Iran

Received: 2024-11-25 Accepted : 2025-04-13 Published : 2025-06-18

Keywords: Exercise training, Diet, Insulin resistance, Lipid profile, Obesity,

Abstract :

Obesity is associated with lipid disorders and a significant increase in cardiovascular diseases. This study aimed to investigate the effect of 10 weeks of high-intensity interval training (HIIT) and high-protein diet (HPD) on the plasma levels of apolipoprotein A1 and B and lipid profile in obese male rats. 40 obese male Wistar rats in five groups (n = 8): HIIT, HPD, HPD+HIIT, obese control-A (OC-A), and obese control-B (OC-B) and eight rats were also studied as normal control group (NC). Training groups, ran five days a week for 10 weeks with at the about intensity of 90% of maximum oxygen uptake on the treadmill. After the end of the intervention, blood sampling was done and the levels of apolipoproteins ApoA1, ApoB, and lipid profile were measured in plasma. The data were analyzed using one-way analysis of variance and Tukey's post-hoc tests at a significance level of p < 0.05. The plasma level of HDL-C was significantly higher in the NC group than in the OC-A group (p ˂ 0.05), but no significant difference was observed between the other groups (p > 0.05). The TC/HDL-C and LDL-C/HDL-C ratios in the NC and HIIT+HPD groups were significantly lower compared with the OC-A group (p ˂ 0.05), while no significant difference was observed between the other groups (p ˃ 0.05). Also, triglyceride-glucose index (TyG) was significantly lower in the NC, OC-B, HIIT, HPD, and HIIT+HPD groups than in the OC-A group (p < 0.05). No significant difference were observed in the plasma levels of ApoA1, ApoB, ApoB/ApoA1, Triglyceride (TG), Total cholesterol (TC), Low-density lipoprotein (LDL-C), and Very low density lipoprotein (vLDL-C) among the groups (p > 0.05). It seems that doing HIIT along with HPD can prevent possible metabolic complications caused by obesity and high-fat diets by improving the lipid profile.

 

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