ZnO-Rutin nanoparticles: a potent formulation to inhibit Staphylococcus aureus biofilm

Subject Areas : Biotechnological Journal of Environmental Microbiology

Fatemeh Azizi Alidoust ¹ , Behnam Rasti ² , Hojjatola Zamani ³ , Mirsasan Mirpour ⁴ , َAmir Mirzaei ⁵

1 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran
2 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran
3 - Department of Biology, University of Guilan, Rasht, Iran
4 - Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran
5 - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran

Received: 2023-11-25 Accepted : 2024-12-01 Published : 2023-12-11

Keywords: Biofilm, Drug resistance, Rutin, ZnO ,

Abstract :

Staphylococcus aureus is a major human pathogen. This opportunistic pathogen is responsible for a variety of infections in humans. Biofilm formation is a key factor in infection development and drug resistance. Biofilm acts as a barrier and reduces bacterial exposure to antibacterial drugs. Targeting bacterial biofilm is a promising strategy to overcome drug resistance. In this work, the antibiofilm potential of ZnO-rutin nanoparticles was characterized. Growth inhibitory potential was studied using the broth microdilution method. Biofilm inhibitory effects of ZnO and ZnO-rutin nanoparticles were investigated by crystal staining and electron microscopy imaging was used to visualize the treated and untreated biofilms. The minimum inhibitory concentration (MIC) of the ZnO and ZnO-rutin nanoparticles for S. aureus strains was 0.5-1.0µg/mL. Treatment of S. aureus with ZnO and ZnO-rutin nanoparticles inhibited biofilm formation by 68.2 and 81.2%, respectively. In addition, exposure of S. aureus with ZnO-rutin nanoparticles considerably disrupted biofilm architecture, inhibited cell adhesion to the surface, and caused morphological alteration of biofilm architecture. Additionally, damage to cell surface was evident in the treated cells. This study shows that ZnO-rutin can be a promising candidate for combating biofilm-associated infection and drug resistance caused by S. aureus.

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