The effect of 8 weeks of aerobic exercise on liver enzymes, and anthropometric indicators of rats exposed to electromagnetic waves
Subject Areas : blood biochemistry & investigation of biomarkers
ommolbanin ghasemian
1
,
asiye seyyed
2
1 -
2 - Department of Sport physiology, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
Keywords: electromagnetic waves, heart weight, ratio of heart weight to body weight, aerobic exercise, liver enzymes,
Abstract :
Considering the impact of physical activity on human health, the present study aims to investigate the effects of 8 weeks of aerobic training on liver enzymes, heart weight, body weight, and the heart weight-to-body weight ratio in rats exposed to electromagnetic waves.
In this experimental study, 32 rats were randomly divided into four groups: (1) a control group without any intervention, (2) a control group exposed to wireless electromagnetic radiation, (3) an aerobic exercise group exposed to wireless electromagnetic radiation, and (4) an aerobic exercise group without electromagnetic radiation (each group has 8 rats). Groups 2 and 3 were exposed to electromagnetic radiation for 1 hour a day over eight weeks. The samples from groups 3 and 4 underwent treadmill exercise three times a week for the same duration. Exposure to wireless radiation had no significant effect on alanine aminotransferase and aspartate aminotransferase levels, but it resulted in increased weight, heart weight, and the heart weight-to-body weight ratio in rats (P<0.05). Aerobic exercises led to decreased weight, heart weight, and the heart weight-to-body weight ratio in rats exposed to wireless radiation (P<0.05). Additionally, aerobic exercise increased alanine aminotransferase and aspartate aminotransferase levels in rats under wireless radiation (P<0.05). Notably, aspartate aminotransferase levels in the aerobic exercise group were significantly higher than those in the aerobic exercise group exposed to wireless radiation (P<0.05).
It seems that electromagnetic radiation has no effect on liver enzymes, while it increases the levels of weight, heart weight and the ratio of heart weight to body weight in rats. Performing aerobic exercises reduces the weight, heart weight and ratio of heart weight to body weight of rats exposed to wireless radiation and has a positive effect on liver enzymes.
Adebayo, E. A., et al. Bio-physical effects of radiofrequency electromagnetic radiation (RF-EMR) on blood parameters, spermatozoa, liver, kidney and heart of albino rats (2019). Journal of King Saud University-Science 31(4), 813-821. 10.1016/j.jksus.2018.11.007
Amiri, H., Shabkhiz, F., Pournemati, P., Quchan, A. H. S. K., & Fard, R. Z. (2023). Swimming exercise reduces oxidative stress and liver damage indices of male rats exposed to electromagnetic radiation. Life Sciences, 317, 121461. https://doi.org/10.1016/j.lfs.2023.121461
Azab, A., & Ebrahim, S. (2017). Exposure to electromagnetic fields induces oxidative stress and pathophysiological changes in the cardiovascular system. Journal of Applied Biotechnology & Bioengineering, 4(2), 00096. https://doi.org/10.15406/jabb.2017.04.00096
Bae, J. C., Suh, S., Park, S. E., Rhee, E. J., Park, C. Y., Oh, K. W., Park, S. W., Kim, S. W., Hur, K. Y., & Kim, J. H. (2012). Regular exercise is associated with a reduction in the risk of NAFLD and decreased liver enzymes in individuals with NAFLD independent of obesity in Korean adults. PLoS ONE, 7(10), e46819. https://doi.org/10.1371/journal.pone.0046819
Baharara, J., PARIVAR, K., Ashraf, A., & MAJIDI, B. (2008). The effects of mobile phone waves (940MHz) on embryonic development of hematopoiesis system in Balb/C mouse. Journal of Shahrekord University of Medical Sciences, 10(1), 1-8. https://sid.ir/paper/58953/en
Baharara, J., & Zahedifar, Z. (2012). The effect of low-frequency electromagnetic fields on some biological activities of animals. Journal of Arak University of Medical Sciences, 15(7), 80-93. http://jams.arakmu.ac.ir/article-1-1144-en.html
Balanejad, S., Parivar, K., Baharara, J., & Mohseni Kochesfahani, H. (2010). Effect of Combinned rapamycine and of low frequency electromagnetic field on angiogenesis. Journal of Shahrekord University of Medical Sciences, 11(3), 70-76.
Bodera, P., Stankiewicz, W., Antkowiak, B., Paluch, M., Kieliszek, J., Sobiech, J., & Niemcewicz, M. (2015). Influence of electromagnetic field (1800 MHz) on lipid peroxidation in brain, blood, liver and kidney in rats. International Journal of Occupational Medicine and Environmental Health, 28(4), 751-759. http://dx.doi.org/10.13075/ijomeh.1896.00255
Cengız, A. (2012). Aerobic training induced structural changes of the heart. Turkish Journal of Sport and Exercise, 14(3), 1-5. https://doi.org/10.15314/tjse.11663
Cooper, C., Vollaard, N. B., Choueiri, T., & Wilson, M. (2002). Exercise, free radicals and oxidative stress. Biochemical Society Transactions, 30(2), 280-285. https://doi.org/10.1042/bst0300280
Daniela, M., Catalina, L., Ilie, O., Paula, M., Daniel-Andrei, I., & Ioana, B. (2022). Effects of exercise training on the autonomic nervous system with a focus on anti-inflammatory and antioxidants effects. Antioxidants, 11(2), 350. https://doi.org/10.3390/antiox11020350
Davoodi, M., Moosavi, H., & Nikbakht, M. (2012). The effect of eight weeks selected aerobic exercise on liver parenchyma and liver enzymes (AST, ALT) of fat liver patients. Journal of Shahrekord University of Medical Sciences, 14(1), 84-90. https://sid.ir/paper/58360/en
Fathi, K., Ghorbani, F., & Mojtahedi, H. (2014). Effect of 6 week aerobic step training on cardiovascular fitness, body composition, flexibility, anaerobic power and quality of life of female students of isfahan university. Iranian Journal of Ergonomics, 2(2), 29-37. http://journal.iehfs.ir/article-1-89-en.html
Fathi, M., & Abroun, S. (2015). The effect of endurance training on left ventricle hp-1β gene expression in wistar male rat. Razi Journal of Medical Sciences, 22(136), 60-67.
Fathi, M., Gharakanlou, R., & Rezaei, R. (2014). The effect of 14-week endurance training on left ventricle HDAC4 gene expression of wistar male rat. Sport and Biomotor Sciences, 6(1 (11)), 5-15. https://sid.ir/paper/205307/en
Fathy Assasa, M. (2010). Effect of cellular phone field on body weight, liver enzymes blood indoces and role of some antioxidant in albino rats. Al-Zahra Assiut Med J, 8(3), 68-83.
Elmas, Onur. "Effects of electromagnetic field exposure on the heart: a systematic review." Toxicology and industrial health, 32.1 (2016): 76-82. https://doi.org/10.1177/07482337134984
Gaeini, A., Kazemi, F., Mehdiabadi, J., & Shafiei-Neek, L. (2012). The effect of 8-week aerobic interval training and a detraining period on left ventricular structure and function in non-athlete healthy men. Zahedan Journal of Research in Medical Sciences, 13(9), e93706.
Galedari, M., & Kaki, A. (2017). The effect of 12 weeks high intensity interval training and resistance training on liver fat, liver enzymes and insulin resistance in men with nonalcoholic fatty liver. Jundishapur Scientific Medical Journal, 16(5), 493-503. https://doi.org/10.22118/jsmj.2017.53990
Ghasemi M, J. M., Sobhani V, Chavoshi F, Rezaei M, Raffati S. (2022). The role of 15-week aerobic exercise training in improving cardiovascular fitness in flight attendants. Journal of Military Medicine, 14(4), 285-292.
Goraca, A., Piechota, A., & Huk-Kolega, H. (2009). Effect of alpha--Lipoic acid on LPS-induced oxidative stress in the heart. Acta Physiologica Polonica, 60(1), 61.
Gul, M., Demircan, B., Taysi, S., Oztasan, N., Gumustekin, K., Siktar, E., Polat, M. F., Akar, S., Akcay, F., & Dane, S. (2006). Effects of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 143(2), 239-245. https://doi.org/10.1016/j.cbpa.2005.12.001
Hasan, I., & Islam, M. R. (2020). Biochemical and histopathological effects of mobile phone radiation on the liver of Swiss albino mice. European Journal of Anatomy, 24(4), 257-261.
Kashani, Z. A., Pakzad, R., Fakari, F. R., Haghparast, M. S., Abdi, F., Kiani, Z., Talebi, A., & Haghgoo, S. M. (2023). Electromagnetic fields exposure on fetal and childhood abnormalities: Systematic review and meta-analysis. Open Medicine, 18(1), 20230697. https://doi.org/10.1515/med-2023-0697
Kavian Nejad, R. H. N., Mohammad Taghi R, Gharibi F. (2009). The effect of mobile phone electromagnetic waves on blood pressure, heart rate and arrhythmia. Scientific Journal of Gorgan University of Medical Sciences, 11(3), 22-26.
Kıvrak, E. G., Yurt, K. K., Kaplan, A. A., Alkan, I., & Altun, G. (2017). Effects of electromagnetic fields exposure on the antioxidant defense system. Journal of Microscopy and Ultrastructure, 5(4), 167-176. https://doi.org/10.1016/j.jmau.2017.07.003
Klabunde, R. E. (2017). Cardiac electrophysiology: normal and ischemic ionic currents and the ECG. Advances in Physiology Education, 41(1), 29-37. https://doi.org/10.1152/advan.00105.2016
Kulkarni, G. A., & Gandhare, W. Z. (2015). Effect of extremely low frequency electric field on liver, kidney, and lipids of Wistar rats. International Journal of Medical Science and Public Health, 4, 1755.
Lai, H. (2019). Exposure to static and extremely-low frequency electromagnetic fields and cellular free radicals. Electromagnetic Biology and Medicine, 38(4), 231-248. https://doi.org/10.1080/15368378.2019.1656645
Lin, J. C. (2012). Electromagnetic fields in biological systems. Taylor & Francis.
Mohammadi, R., Matin Homaee, H., Azarbayjani, M., & Baesi, K. (2017). The Effects of 12 week Endurance Training on glucose amount, Blood insulin and Heart Structure in type 2 diabetic Rats. Community Health Journal, 9(3), 29-36.
Monsefi, M. (2008). Stereological study of heart volume in male rats after exposure to electromagnetic fields. Iranian South Medical Journal, 10(2), 112-118. http://ismj.bpums.ac.ir/article-1-15-en.html
Moosavi-Sohroforouzani, A., & Ganbarzadeh, M. (2016). Reviewing the physiological effects of aerobic and resistance training on insulin resistance and some biomarkers in non-alcoholic fatty liver disease. KAUMS Journal (FEYZ), 20(3), 282-296. http://feyz.kaums.ac.ir/article-1-3091-en.html
Moriles, K. E., & Azer, S. A. (2020). Alanine amino transferase. StatPearls Publishing, Treasure Island (FL).
Peighambarzadeh, S. Z., & Tavana, M. (2017). Effects of electromagnetic field radiation on biochemical parameters in swiss albino mice. Banat's Journal of Biotechnology, 8(16), 48-53. https://doi.org/10.7904/2068-4738-VIII(16)-48
Pooladi, M., Montzeri, A., Nazarian, N., Taghizadeh, B., & Odoumizadeh, M. (2018). Effect of WiFi waves (2.45 GHz) on aminotransaminases (ALP, ALT and AST) in liver of rat. Archives of Advances in Biosciences, 9(2), 13-20. https://doi.org/10.22037/jps.v9i2.19283
Pourfazeli, B., Azamian Jazi, A., Faramarzi, M., & Mortazavi, M. (2017). Effect of eight weeks aerobic training on oxidative stress markers in rats exposed to electromagnetic microwave radiation emitted from wi-firouters. Armaghane Danesh, 22(3), 311-324. http://armaghanj.yums.ac.ir/article-1-1834-en.html
Rajaei, F., & Mohammadian, A. (2013). Effects of extremely low frequency electromagnetic field on mouse liver histology. Qom University of Medical Sciences Journal, 6(4 (24)), 8-13. https://sid.ir/paper/132225/en
Rashidi S, A. F. (2017). Investigating the effect of electromagnetic waves emitted from mobile phones on liver activity and some transaminases in mouse blood serum. Knowledge Reference, 2(5), 20-35.
Rawlins, J., Bhan, A., & Sharma, S. (2009). Left ventricular hypertrophy in athletes. European Journal of Echocardiography, 10(3), 350-356. https://doi.org/10.1093/ejechocard/jep017
Rezaie, M. (2013). Effect of electromagnetic waves generated by base transiver station on liver enzymes in female rats. Zahedan Journal of Research in Medical Sciences, 15(7), 19-21.
Sabban, I. F., Pangesti, G., & Saragih, H. T. (2018). Effects of Exposure to Electromagnetic Waves from 3G Mobile Phones on Oxidative Stress in Fetal Rats. Pakistan Veterinary Journal, 38(4), 384-388. http://dx.doi.org/10.29261/pakvetj/2018.094
Sani, A., Labaran, M., & Dayyabu, B. (2018). Effects of electromagnetic radiation of mobile phones on hematological and biochemical parameters in male albino rats. European Journal of Experimental Biology, 8(2), 11. http://dx.doi.org/10.21767/2248-9215.100052
Shamsoddini, A., Sobhani, V., Chehreh, M. E. G., Alavian, S. M., & Zaree, A. (2015). Effect of aerobic and resistance exercise training on liver enzymes and hepatic fat in Iranian men with nonalcoholic fatty liver disease. Hepatitis Monthly, 15(10), e31434. http://dx.doi.org/10.5812/hepatmon.31434
Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012(1), 217037. https://doi.org/10.1155/2012/217037
Siddiqi, Najam, et al. "Mobile phone electromagnetic waves causing fatty change in the hepatocytes of the developing chick embryo: Are smart phones too close for comfort?." Biomedical and Pharmacology Journal, 10.3 (2017): 1139-1147. https://doi.org/10.13005/bpj/1214
Silva, M., Santos, F., Lagares, L., Macedo, R., Takanami, L., Almeida, L., Bomfim, E., & Santos, C. (2023). Physical exercise and changes in ast/alt rates in non-alcoholic fatty liver disease: a systematic review of clinical trials. Preprints, 2023, 2023040318. https://doi.org/10.20944/preprints202304.0318.v1
Stevens, R. G. (2004). Electromagnetic fields and free radicals. Environmental Health Perspectives, 112(13), A726-A726. https://doi.org/10.1289/ehp.112-a726a
Usikalu, M. R., et al. (2010). Low level microwave exposure decreases the number of male germ cells and affect vital organs of Sprague Dawley rats. Am. J. Sci. Ind. Res 1, 410-420. 10.5251/ajsir.2010.1.3.410.420
Valizadeh, R., Nikbakht, M., Davodi, M., & Khodadoost, M. (2011). The effect of eight weeks elected aerobic exercise on the levels of (AST, ALT) enzymes of men patients with have fat liver. Procedia-Social and Behavioral Sciences, 15, 3362-3365. https://doi.org/10.1016/j.sbspro.2011.04.300
Zinvand Lorestani, A., & Rahmati, M. (2018). The effect of eight weeks of aerobic training on the levels of enzymes associated with non-alcoholic fatty liver in obese children. Yafteh, 20(2), 53-61.
