The effect of a course of crosetin consumption on LC3 gene expression in heart tissue of elderly diabetic rats following intermittent aerobic exercise

Subject Areas : Open Access

Abolhasan Sharifi Rigi ¹ , khosro jalali dehkordi ² , Mohsen Akbarpour Beni ³ , Farzaneh Taghian ⁴

1 - Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Associate Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
3 - Associate Professor, Department of Physical Education and Sport Sciences, University of Qom, Qom, Iran
4 - Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Received: 2024-09-05 Accepted : 2024-10-20 Published : 2024-09-18

Keywords: Aerobic exercise, crosetin, LC3, heart, elderly, diabetes,

Abstract :

Introduction: Performing sports activities is one of the most important basic strategies to control and treat blood glucose and hyperlipidemia, and it reduces the incidence of diabetes and cardiovascular complications. The purpose of this study is to investigate aerobic exercise and the effective substance crostin on tissue LC3 gene expression. The heart was an experimental model of diabetic rats.

 

Materials and Methods: In this experimental trial, 25 elderly female c57bl6 male mice with diabetes (40 mg/kg peritoneal injection) aged 14-16 weeks and weighing 30-35 grams were divided into 5 groups: healthy control, aerobic exercise + crosetin, diabetic, diabetic + aerobic exercise. Diabetic + crosetin, diabetic + aerobic exercise + crosetin were divided. Intermittent aerobic exercise was performed for eight weeks, five sessions per week. Rats received crosetin 30 mg/kg/day by intraperitoneal injection. LC3 expression was measured by Real Time PCR method. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used (P≥0.05).

 

Findings: LC3 gene expression was significantly increased in the diabetic group compared to the healthy group (P=0.001). LC3 gene expression in the group of diabetes+aerobic exercise+crosetin supplement, diabetes group+aerobic exercise and diabetes and crosetin supplement was significantly decreased relative to the diabetes group (P=0.001).

 

Conclusion: It seems that intermittent aerobic exercise and crosetin both alone and simultaneously decreased LC3 in diabetic heart tissue. Therefore, it is recommended to use intermittent aerobics and crosetin along with therapeutic modalities in type 2 diabetes in old age.

 

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