Development of antibiotic resistant mutants of some antagonistic bacteria and investigation of their antagonistic activity and mechanisms against Rhizoctonia solani, the causal agent of cotton seedling damping-off

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

H. Jahanifar ¹ , A. Heydari ² , Nader Hasanzadeh1 ³ , L. Naraghi ⁴

1 - کارشناس ارشد بیماری شناسی گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد علوم و تحقیقات.
2 - استادیارمؤسسه تحقیقات گیاه پزشکی کشور.
3 - دانشیارگروه بیماری شناسی گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد علوم و تحقیقات.
4 - مربی پژوهش مؤسسه تحقیقات گیاه پزشکی کشور.

Received: 2013-11-05 Accepted : 2013-11-05 Published : 2013-09-23

Keywords: Rhizoctonia solani, Antagonistic bacteria, antibiotic resistant mutants, inhibitory mechanisms, cotton seedling damping-off,

Abstract :

In this study, antibiotic resistant mutants of antagonistic bacteria to Rifampicin and Nalidixic acid using spontaneous mutation were obtained. Antagonistic activity of three isolates belonging to Pseudomonas fluorescens were tested in vitro against a virulent isolate of Rhizoctonia solani, the causal agent of cotton seedling damping-off, using dual culture method. It was shown that two isolates had better antagonistic traits against fungus in comparison with their wild types. The above isolates were also evaluated for their inhibitory mechanisms against fungal pathogen and the results showed that all three mutants were different than their wild types in volatile metabolites production. There were no differences between the mutants and wild types in production of non-volatile metabolites. However, some mutants (Pf-C-1, Pf-W-2) produced more siderophores than their wild types. Results of this study suggest that spontaneous mutation may affect and enhance the activity and performance of antagonistic bacteria.

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