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Article Type: Original Research

Study on antagonistic efficacy of root endophytic Fungi and Trichoderma species on Macrophomina phaseolina in vitro

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

Fariba Abbaszadeh 1 (گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.)
Ebrahim Mohammadi Goltapeh 2 (گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.)
E. Pourjam 3 (گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.)
Amir Khorasani 4 (گروه بیماری شناسی گیاهی دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران ایران.)
Y. Razaei Danesh 5 (گروه گیاهپزشکی ، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه ایران.)
A. Varma 6 (گروه مطالعاتی گیاهان داروئی و میکروبی، دانشگاه آمیتی نویدا ایالت اترپرادش، هندوستان.)

Received: 2013-11-02 Accepted : 2013-11-02 Published : 2013-09-23

Keywords: Trichoderma spp, Sebacina vermifera, biological control, Piriformospora indica, Macrophomina phaseolina,

Abstract :

Two root endophytic fungi (Piriformospora indica Varma and Sebacina vermifera Warcup), as well as two species of Trichoderma (T. viride and T. harzianum (T-100)) were used against Macrophomina phaseolina. Antagonistic ability of root endophytic fungi and Trichoderma species against the pathogen in dual culture, volatile metabolites and colonization were evaluated. Assays in dual culture revealed that antagonistic fungi produced a good zone of inhibition. Interaction of root endophytic fungi and Trichoderma species in combination resulted in maximum inhibition of mycelial growth and microsclerotia formation of the pathogen. The volatile metabolite studies revealed that endophytic fungi did not act by producing volatile metabolites but possibly by other mechanisms of competition or parasitism. As for mechanism of colonization, it was revealed that antagonistic fungi were able to overgrow the colony of the pathogen and lyse its mycelia.

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