Identification Leptosphaeria maculans and Leptosphaeria biglobosa with some in vitro morphological ‎characteristics ‎

Subject Areas : دو فصلنامه تحقیقات بیماریهای گیاهی

ali zaman mirabadi ¹ (Chief of Applied Research& Seed Production Oilseeds Research & Development Company)
[e-mail] Kamran Rahnama ² (Associate Professor, Department of Plant Protection, Gorgan University of Agricultural Sciences & Natural Resources, Gorgan, Iran)
Mehdi Sadravi ³ (Associate Professor, Department of Plant Protection, Yasouj University, Yasouj, Iran)
Mansoor Salati ⁴ (Research Assistant Professor, Agricultural and Natural Resources, Research Center of Khorasan Razavi, Mashad, Iran.)

Received: 2018-02-20 Accepted : 2020-12-24 Published : 2020-05-21

Keywords: Leptosphaeria maculans, blackleg, L.biglobosa, medium culture and oilseed rape,

Abstract :

This study has carried out for distinguishing of Leptosphaeria maculans and L. biglobosa (aggressive and ‎non-aggressive groups respectively) using colony growth, ability for pycnidium and pigment production in four ‎mediums and temperatures of Forty nine isolates (Zaman Mirabadi et al., 2010) (totally 784 treatments) in three ‎replication and three times. The result of analysis variation has shown that significant difference between isolates ‎considering colony growth in the different mediums and temperature treatments. The maximum and the minimum ‎colony growth was observed in 30⁰c –v8 juice Agar (48.05 mm) medium and 15⁰c-Czapek Dox Agar (17.01 mm) ‎respectively after 12 days. The greatest and lowest pigment production in the different temperature was observed in ‎PDA and MEA (except in 30⁰c). The minimum and maximum pigment production was observed in 15⁰c and 30⁰c ‎respectively. The percentage of presence yellow-orange and brown pigment was‏ ‏variable in the different treatments. ‎Brown pigment in V8 and CDA was dominated while yellow- orange was seen in PDA and MEA. 93.78 % isolates ‎produced pigment in Czapek Dox Broth. The maximum pycnidium production was observed in V8 and 20⁰c. ‎According to the obtained results, Dasht-Naz, Eslamabad, Fazelabad, Kohi khail and bykar ayesh were belonged to ‎aggressive species (L.maculans) and Talokola, Lala, Khalkhail and mustard isolate too were located as L.biglobosa

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