Effects of galanin-like peptide on plasma cortisol levels and feed intake of broiler chickens: focusing on the role of serotonergic receptors
Subject Areas : Experimental physiology and pathology
Elham Sanadgol
1
,
Morteza Zendehdel
2
,
Bita Vazir
3
,
Ali Rassouli
4
,
Hadi Haghbin nazarpak
5
1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5 - 5- Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Galanin-like peptide, Serotonergic receptors, Appetite, Broiler,
Abstract :
Background & Aim: In the studies conducted on mammalian models, the role of galanin-like peptide (GALP) in some physiological processes such as the regulation of food intake has been determined. In this regard, in the present study, while evaluating the effects of central injection of GALP on the plasma cortisol level, we discuss its interaction with serotonergic receptors in the regulation of food consumption in broiler chickens.
Materials and methods: 176 broiler chickens were divided into groups of 11 to perform four experiments. In the first experiment, GALP was administered in doses of 0.5, 1 and 2 μg. In the second experiment, fluoxetine, GALP and fluoxetine + GALP were injected. The third and fourth tests were similar to the second test, with the difference that 8-OH-DPAT (5-HT1A receptor agonist) and SB242084 (5-HT2C receptor antagonist) were replaced by fluoxetine, respectively. After the chicks returned to their cages, the cumulative food intake was recorded at 30, 60 and 120 minutes after injection. Finally, by cutting the head, blood was collected and plasma cortisol levels were measured in chickens receiving GALP.
Results: Based on the findings, injection of GALP in doses of 1 and 2 μg significantly increased food intake in broilers (P<0.05). Also, although the administration of 8-OH-DPAT + GALP did not significantly change the hyperphagia induced by GALP, the simultaneous injection of fluoxetine and SB242084 with GALP inhibited this hyperphagic effect (P<0.05). Administering different doses of GALP also had no significant effect on plasma cortisol levels (P≤0.05).
Conclusion: The results of this study showed that the hyperphagic effect of GALP is probably mediated through 5-HT2c receptors in broilers.
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