Comparison of the therapeutic effects of amniotic membrane mesenchymal stem cells, directly and indirectly, in renal failure induced by acute cardiac ischemia in male Wistar rats.
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganismsamir akbari 1 , Mahsa Ale-Ebrahim 2 , noushin barik rou 3 , fateme roholah 4
1 - Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3 - Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
4 - 1 Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Keywords: Mesenchymal stem cells, amniotic membrane, acute heart ischemia, renal failure,
Abstract :
Cardiac ischemia and subsequent renal failure are very common. The researchers found that with the transfer of stem cells, dead tissues can be replaced and cause the damaged parts of the heart and thus the kidney tissue to function again. Materials and methods: Mesenchymal stem cells (MSCs) of amniotic membrane were analyzed by flow cytometry. The rats were divided into 3 groups of 12, including heart failure (HF) as a control , HF+ hAMSCs injected into the heart, and HF+ hAMSCs injected into the kidney. Then, with LAD ligation , an acute cardiac ischemia was created in rats and cells were injected into the damaged heart and kidney tissue separately, after 2 and 30 days using IHC, TNF-𝛼 in kidney tissue , urea and creatinine serum levels was investigated. Results and discussion: the effects of the cells on the heart and kidneys of rats were investigated, according to the IHC results, the level of TNF-𝛼 protein was significantly decreased on day 30, in the group of cells injected into the kidney compared to the control (P<0.05). There was a significant difference in the serum urea and creatinine between the kidney cell injection and control on day 30 (P<0.05). Treatment with MSCs on the 2nd day after the induction of cardiac ischemia didn't have a significant therapeutic effect compared to the control, but on the 30th day, the kidney cell injection group, were able to reduce inflammation, improve the damaged area and decrease Fibrosis in kidney tissue.
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