The effect of irrigation system and two variants of PGPR on phenolic compounds and antioxidant activity of saffron stigma extract
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
reza dehghani
1
(university of kashan)
Keywords: Flavonoids, PGPR, Drip irrigation, Extract, Saffron,
Abstract :
Phenolic compounds (flavonoids, tannins, and anthocyanin) are considered as most important natural antioxidants and many factors such as environmental parameters and plant nutrition affect their quantity and quality. One of the native medicinal plants of Iran is saffron with the scientific name of Crocus sativus Var. officinalis. That the use of its compounds be traced to ancient times and there is natural and cultivated in the different regions of the country. This study is done with aims to evaluate the quality and quantity of phenolic compounds and antioxidant activity Saffron species under the influence of two types of PGPR (Azotobacter chroococcum 5 and pseudomonas fluorescens 169) and two irrigation systems (flooding and drip). In this study, plant phytochemistry study was conducted initially, and then measuring a number of phenolic compounds and flavonoids were spectrophotometry method. Finally, the antioxidant activity of the extract was measured in different concentrations using free radical scavenging (DPPH). Data analysis was performed by using SPSS version 19 software and ANOVA test. The results of phytochemical tests confirmed the compounds such as tannins, anthocyanins and flavonoids and absence of alkaloids. A number of flavonoid compounds in the sample with Azotobacter chroococcum 5 was more than other samples. pseudomonas fluorescens 169 and flooding treatments had the highest and lowest number of phenolic compounds and drip irrigation showed the highest antioxidant properties. Therefore, the use of growth-stimulating bacteria and replacing the drip irrigation system in saffron cultivation is suggested
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