inoculated with Rhizobium leguminosarum bv phaseoli and plant growth promoting Rhizobacteria (PGPR)

Subject Areas : Agroecology Journal

Mehrab Yadegari ¹ (Assist Professor of Agriculture Faculty, Islamic Azad University, Shahrekord Branch.)
ghorban normohammadi ² (Professor in Agronomy, Faculty of Agronomy, Islamic Azad University of Tehran, Science and Research Branch.)
hadi Asadi Rahmani ³ (Assist Professor of Soil and Water Research Institute, Tehran.)

Received: 2008-05-14 Accepted : 2009-03-03 Published : 2009-08-23

Keywords: yield, Yield components, Red bean, Phaseolus vulgaris L, Rhizobium leguminosarum biovar phaseoli, Plant growth promoting rhizobacteria,

Abstract :

The effect of co-inoculation with plant growth promoting Rhizobacteria (PGPR) and Rhizobium on yield and yield components of red bean, Phaseolus vulgaris L. cultivars was investigated in agricultural research station of Islamic Azad university of Shahrekord during 2006-2007. The experiment was done in split plot in the basis of randomized complete block design in three replications. Six strains of Rhizobium and a nitrogen fertilizer treatment were considered as the main factor and three red bean cultivars (Akhtar, Gholi, and Sayyad) were as the sub factors. PGPR strains of Pseudomonas fluorescens P-93 and Azospirillum lipoferum S-21 as well as two highly effective Rhizobium strains were used in this study. Common bean seeds of three cultivars were inoculated with Rhizobium singly or in  combination with PGPR to evaluate their effect on growth characters. Results indicateed that soil beneficial bacteria can positively affect symbiotic performance of Rhizobia. A significant variation of plant growth in response to inoculation with Rhizobium strains was observed. Treatment with PGPR significantly increased number of pods per plant, number of seeds per pod, weight of seeds per plant, weight of pods per plant, total dry matter in R6 stage as well as seed yield and protein content. Co-inoculation with Rhizobium and PGPR caused a significant increase in the yield and yield components. The results showed that all treatments of bacteria increased yield. However, strains Rb-136 with Pseudomonas fluorescens P-93 caused the highest seed yield, number of pods per plant, weight of 100 seed, number of seeds per pod and seed protein yield. 

References:

1.              مدیر شانه چی، م.1371. تولید و مدیریت گیاهان علوفه‏ای. انتشارات آستان قدس رضوی،235-232.

2.              یادگاری، م.، غ. اکبری وج. دانشیان.1382. اثرات تلقیح چهار سویه باکتری بر عملکرد و اجزای عملکرد سویا در شرایط آب و هوایی کرج. مجله علمی پژوهشی پژوهش‌های زراعی ایران، دانشگاه فردوسی مشهد، جلد1، شماره 1، ص. 93-109.

3.     Asadi Rahmani, H., and Rastin, N. S. 2000. Prediction of the necessity of soybean inoculation based on the numbers of Bradyrhizobium japonicum. Ninth Congress of African Association for Biological N₂ Fixation, 65.

4.     Bremer E., and Kessel, C. V. 1990. Selection of leguminosarum strains for lentil under growth room and field condition.Plant and Soil 121:47-56.

1. Dey. R., Pal, K. K., Bhatt, D. M., and Chauhan, S. M. 2004. Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growthpromoting Rhizobacteria. Microbiological Research 159: 371-394.


2. Galal, G. M. 1997. Dual inoculation with strains of Bradyrhizobium japonicom and Azospirillum brasilense to improve growth. Biology and Fertility of Soils 24: 317-322.


3. Gray, E. J., and Smith, D. L. 2005. Intracellular and extra cellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biology Biochemistry 37: 395-410.


4. Hungria, M., and Bohrer, T. R. J. 2000. Viability of nodulation and dinitrogen fixation capacity among soybean cultivars. Biology and Fertility of Soils 31:45-52.


5. Maingi, J. M., Shisanya, C. A., and Gitonga, N. M. 2001. Nitrogen fixation by common bean (Phaseolus vulgaris L.) in pure and mixed stands in semiarid south – east Kenya. European Journal of Agronomy 14:1-12.

10.Ravi, S., Gadagi, P. U., Krishna, K. J. H., and Tongmin S. A. 2004. The effect of combined Azospirillum inoculation and nitrogen fertilizer on plant growth promotion and yield response of the blanket flower Gaillardia pulchella. Scientia Horticulture 100: 323–332.

11.Rodriguez –Navarro. D. N., Buendia, A. M., Camacho, M., and Lucas, M. M. 2000. Characterization of Rhizobium spp. bean isolates from southwest Spain. Soil Biology and Biochemistry 32: 1601 –1613.

12.Senevirante, G., Vanholm, L. H. J., and Ekanayake, E. M. H. G. S. 2000. Agronomic benefits of rhizobia inoculants use over nitrogen fertilizer application in tropical soybean. Field Crops Research 68: 199-203.

13.Tilak, N., Ranganayaki, F., and Manoharachari, C. 2006. Synergistic effects of plant-growth promoting Rhizobacteria and Rhizobium on nodulation and nitrogen fixation by pigeon pea (Cajanus cajan). European Journal of Soil Science 57: 67–71.

14.Vederia, L. B., Pastorino, G. N., and Balatti, P. A. 2001. Incompatibility may not be the rule in the sinorhizobium fredii –soybean interaction. Soil Biology and Biochemistry 33: 837-840.

15.Vessey, J. K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255: 571-586.