The study of seed viability, amount of proline and chlorophyll of local genotypes of rice under salt stress

Subject Areas : Agroecology Journal

Shahin Mardani nezhad ¹ , Mansoureh Vazirpour ²

1 - Scientific Board and Member of Young Researchers Club, Islamic Azad University, Mobarakeh Branch
2 - Research Expert in Biology

Received: 2006-07-29 Accepted : 2007-11-15 Published : 2007-09-23

Keywords: Rice, Chlorophyll, Proline, Local genotypes, Salt stress,

Abstract :

Changes of seed germination percentage and proline and chlorophyll amounts of Nogoran and Sorkheh and their known cultivars including, Zayandehrud, Sazandegi, and purred lines of 67-47 and 67-97 in reaction to 0-10 dsm/m sodium chloride concentrations were investigated to compare salinity tolerance of local rice genotypes. Experiment was done in a randomized complete block design with three replications. Analysis of variance and comparison of means were also done with Duncan multiple range test at 1% probability level. Increasing of sodium chloride's concentration in environment decreased seed germination percentage, radicle and coleoptile dry weight and chlorophyll amount of local rice genotypes at 1% probability level. Results of comparison of seed germination percentage means showed better performance of 67-47, Nogoran, Sorkheh and 67-97 than other genotypes. However, dry weight of radicle and coleoptile and amount of chlorophyll were seen in higher rate in genotype 67-47. Amount of proline amino acid in all genotype was increased with increasing of sodium chloride concentration. The highest amount of root and leaf proline was belonged to Sorkheh. Cultivation of genotype 67-47 was recommended in attention to relative salinity of soil in studied region and planting of 67-97 was not recommended duo to sensitivity of it’s chloroplasts to high sodium chloride concentrations.

References:

1- باقریه نجار، م. ب. 1373. بررسی اثر متقابل عامل شوری و ماده ی کلروکلین کلراید بر میزان رشد و جذب فسفر در گیاه برنج. پایاننامه ی کارشناسی ارشد، دانشکده علوم، دانشگاه تربیت مدرس.

2 - شهدی کومله، ع. 1373. بررسی تأثیر منبع و سطوح شوری و میزان ازت بر رشد و ترکیب شیمیایی دو رقم برنج .Oryza Sativa L. پایاننامه ی کارشناسی ارشد، دانشگاه شیراز.

3- صالح، ج. 1378. تأثیر سطوح شوری و منبع روی بر رشد و ترکیب شیمیایی برنج و باقلا. پایاننامه ی کارشناسی ارشد، دانشگاه شیراز.

4- عابدی، ح. 1377. برنج. نشریه تحقیقی ترویجی، کتاب سوم، انتشارات سازمان کشاورزی استان اصفهان.

5- فراست، م. 1373. بررسی تأثیر تنش شوری و دما بر جوانهزنی و مراحل اولیه ی رویش ارقام برنج. پایاننامه ی کارشناسی ارشد علوم گیاهی، دانشگاه تربیت معلم تهران.

6-کاوسی، م. 1374. تعیین مدل مناسب پیشبینی عملکرد برنج در شوریهای مختلف سپیدرود، حسن سرایی و خزر. پایاننامه ی کارشناسی ارشد، دانشگاه شیراز.

7- Ashraf, M. M., and I. Naqvi. 1992. Effect of varying Na+/Ca2+ ratios in salin sand culture on some physiological parameters of four Brassica species. Acta Physiological Plantarum 14:197-205.

8- Bar-Num, N., and A. Polijakooff-Mayber. 1997. Salinity stress and the content of proline in roots of Pisum sativum and Tamarix teragyna. Ann. Bot. 41:173-179.

9- Bates, L. S., R. P. Waldren, and I. D. Tear. 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.

10- Brayan, J. K. 1990. The biochemistry of plants. Advances in the biochemistry of amino acid biosynthesis. Academic Press, New York, 161-196.

11- Brunisma, J. 1961. A Comment on the spectrophotometric determination of chlorophyll. Biochem Biophys Acta 52:576-578.

12- Frederik, L. D., J. P. Billard, J. L. Saos, and C. Hualt. 1993. Effect of NaCl and gabaculine on chlorophyll and proline levels during growth of radish cotyledons. Plant Physiol. Biochem. 31(3): 303-310.

13- Greenway H. and R. Munns. 1980. Mechanisms of salt tolerance in non halophytes. Annual Review of Plant Physiology, 31, 149-190.

14- Khan M. S. A., A. Hamid, and M. A. Karim. 1997. Effect of sodium chloride on germination and seedling characters of different types of rice (Oryza sativa L.). J. Agron. Crop Sci. 179: 163-169.

15- Khatun S., and T. J. Flowers. 1995. Effects of salinity on seed set in rice. Plant Cell Environ. 18: 61-67.

16- Khatun, S., C. A. Rizzo, and T. J. Flowers.1995. Genotypic variation in the effect of salinity on fertility in rice. Plant Soil 173: 239-250.

17- Lin, C. C., and C. H. KAO. 1995. NaCl stress in rice seedlings Effects of L-proline,
 L-glycinebetaine, and D-asparagine on seedling growth.Biological Plantarum 37(2): 305-308.

18- Moons, A., G. Bauw, E. Prinsen, M. M.Van Montagu, and D.V.D. Straeten. 1995. Molecular and physiological responses to abscisic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. Plant Physiol. 107: 177-186.

19- Roy, D., N. Basu, A. Bhunia, and S. K. Banerjee. 1993. Counteraction of exogenous
 L-proline with NaCl in salt sensitive cultivar rice. Biol Plant. 35: 69-72.

20- Saleki, R., P. G. Young, and D. D. Lefelovre. 1993. Mutants of Arabidopsis thaliana capable of germination under saline conditions.Plant Physiol.101:839-845.

21- Trinchart, J. C., Y. S. Yang, and J. Riguad. 1998. Proline accumulation inside symbiosomes of Faba bean nodules under salt stress. Physiological Plantarum 104-38-49.

22- Yoshida, S. F., D. A. Cock, and J. H. Gomez. 1976. Laboratory manual for physiological studies of rice, ed. 3. InternationalRice Research Institute. The Philippines.