Evaluation of different strains of Rhizobium leguminosarum bv. phaseoli inoculation on ecophysiological nitrogen fixation ability of common bean, Phaseolus vulgaris L., cultivars

Subject Areas : Agroecology Journal

Mahdi Taherkhani ¹ (Ph.D Student of Islamic Azad University of Tehran, Science and Research Branch)
ghorban normohammadi ² (Professor in Agronomy, Faculty of Agronomy, Islamic Azad University of Tehran, Science and Research Branch)
mohammadjavad Mirhadi ³ (Assistant Professor in Agronomy, Faculty of Agronomy, Islamic Azad University of Tehran, Science and Research Branch)
hosein Haydari Sharif Abad ⁴ (Associate Professor of Seed and Plant Improvement Institute, Karaj, Iran.)
amirhosein Shirani Rad ⁵ (Assistant Professor of Seed and Plant Improvement Institute, Karaj, Iran)

Received: 2008-04-03 Accepted : 2009-05-18 Published : 2009-04-21

Keywords: yield, common bean, Nitrogen fixation, Inuculant, Rhizobium leguminosarum bv. Phaseoli,

Abstract :

 In order to evaluation of ecophysiological nitrogen fixation ability of common bean (Phaseolus vulgaris L.) cultivars with some types of inoculants which contain different strains of Rhizobium leguminosarum bv. Phaseoli, a factorial experiment in randomized complete block design (RCBD) was conducted in Khoramdareh region of Iran during 2006-2007. Treatments consisted of three types of biological fertilizers including Rb117, Rb123, Rb13, a comercial inoculant (Rhizobean super plus) with two N100 (use of 100 Kg nitrogen/ha) and control (without seeds inoculation and fertilizer application) treatments and three bean cultivars of COS16 (spotted bean), Derakhshan and Akhtar (red beans). Results showed that inoculation of different bean varieties with Rhizobium strains incresead their qualititative and quantititative traits. Inoculation and nitrogen application increased yield up to 43 percent in comparison to control. Rb117 was more effective on common bean yield than other strains and caused up to 59 percent seed yield increase in comparison to non inoculant treatment. Whereas Rb123 had the least effect on yield (32%) among studied inoculants. COS16 was also the best cultivar in most traits and seed yield of this variety was 7.5 and 19 percent higher than Derakhshan and Akhtar varieties, respectively. The most protein percent were obtained from 100 kg/ha nitrogen, and Rb117 treatment. The highest and lowest protein yields were gained from Rb117 and control treatments, respectively. It was concluded that industrial biofertilizers such as Rhizobean super plus must be used as complementary materials with mineral fertilizers to achieve satisfying yield and sustainable agriculture.  

References:

1- اسدی رحمانی، ه.، م. افشاری، ک. خاوازی و ه. سجادی. 1378. بررسی کارآیی تثبیت نیتروژن سویه ریزوبیوم بومی ایران هم‏زیست با گیاه لوبیا. مجله علوم خاک و آب، مؤسسه تحقیقات خاک و آب وزارت جهاد کشاورزی، ص. 97-93.

2- ترابی جفرودی، آ.، ا. فیاض مقدم و ع. حسن‏زاده قورت تپه. 1384. بررسی اثرات تراکم‌ بوته و آرایش‏های مختلف کاشت بر عملکرد دانه و  درصد پروتئین دانه در ارقام لوبیا قرمز تحت شرایط آبی. مجموعه مقالات اولین همایش حبوبات ایران، مشهد، ص. 53.

3- خسروی، ه.، ک. خاوازی و ک. میزاشاهی. 1380. استفاده از مایه تلقیح باقلا به جای کود شیمیایی اوره در منطقه صفی آباد دزفول. مجله علوم خاک و آب (ویژه‏نامه مصرف بهینه کود)، جلد 12 (شماره14)، صفحات 153-146.

4- دادیور، م.، و م. ع. خودشناس. 1384. ارزیابی کارایی مایه تلقیح ریزوبیوم در مناطق عمده لوبیاکاری استان مرکزی. مجموعه مقالات اولین همایش حبوبات ایران، مشهد، ص.830.

5- قاسمی پیر بلوطی. ع و ا. ر. گل‏پرور. 1384. بررسی برخی صفات فیزیولوژیکی و مورفولوژیکی ارقام لوبیا معمولی در منطقه شهرکرد. اولین همایش ملی حبوبات ایران. مشهد. 29 و 30. آبان، ص. 125.

6- مجنون حسینی، ن. 1387. زراعت و تولید حبوبات. جهاد دانشگاهی تهران، چاپ چهارم، 283 ص.

7- همتی، ا. و ه. اسدی رحمانی. 1384. بررسی اثرات تلقیح  سویه‏های ریزوبیوم و مصرف نیتروژن در عملکرد و پروتئین لوبیا چیتی. اولین همایش ملی حبوباتایران، مشهد، ص.223. 

8- یادگاری، م.، ع. قاسمی پیربلوطی، ا. دادی، غ.  ع. اکبری و ا. ر. گل‏پرور. 1384. بررسی توان تثبیت نیتروژن توسط ایزوله‏های مختلف باکتری ریزوبیوم فازئولی در لوبیا چیتی و قرمز در منطقه شهرکرد. مجموعه مقالات اولین همایش حبوبات ایران، مشهد، ص. 152.

9. Babiker, E. E., Elsheikh, A. E., Osman, A. J. and El Tinay, A. H. 1995. Effect of nitrogen fixation, nitrogen fertilization and viral infection on yield, tannin and protein contents and in vitro protein digestibility of faba bean. Plant Foods For Human Nutrition 47:257-263.

10. Beck, D. P., Materon, L. A. and Afandi, F. 1993. Practical Rhizobium- Legume Technology Manual. ICARDA. Technical Manual No.19.

11. Berg R. K. and Lognachan, J. K. 1988. Nodule occupancy by introduced Bradyrhizobium japonicum in zowa soils. Agronomy Journal 80: 876-881.

12. Bremer, E., Kessel, C. V. 1990. Selection of leguminosarum strains for lentil under growth room and field condition. Plant and Soil 121: 47-56.

13. Carter, J. M., Gardner, W. K. and Gibson, A. H. 1994. Improved growth and yield of faba beans (Vicia faba cv. Fiord) by inoculation with strins of Rhizobium leguminosarum biovar. viciae in acid soils in south-west Victoria. Australian  Journal of Agricultural Research 94:613-623.

14. Duong, T. P., Diep. V. C. N., Khiem, N. H., Tol, N. V. and Nhan, L. T. K. 1984. Rhizobium inoculant for soybean [ Glycine max L. Merril] in Mekong dalta. II. Response of soybean to chemical nitrogen fertilizer and Rhizobium inoculation. Plant and Soil. 79:241-247.

15. Elsheikh, E. A. E. and Elzidany, A. A. 1997. Effects of Rhizobium inoculation, organic and chemical fertilizers on yield and physical properties of faba bean seeds. Plant Foods For Human Nutrition 51:137-144

16. Hardarson, G., Danso, S. K. A., Zapata, F. and Reichardt, K. 1991. Measurments of nitrogen fixation in faba bean at different N fertilizer rates using the 15N isotope dilution and A-Value methods. Plant and Soil 131:161-168.

17. Imsand, J. 1992. Agronomic characteristics that identify high yield and high protein soyban genotypes. Agronomic Journal 84: 12-15.

18. Luis Materon, A. and Ryan, J. 1995. Rhizobial inoculation and phosphores and zinc nutrition for annual adupted to mediteranean environments. Agronomy Journal 87: 695-697.

19. May S. N. and Bohlool, B. 1983. Competition among Rhizobium leguminosarum strains for nodulation of lentils. Applied Microbiology 45: 960-965.

20. McKenzie. R. H., Middleton, A. B., Seward, K. W., Gaudiel, R., Wildschut, C. and Bremer, E. 1992. Fertilizer responses of dry bean in southern Alberta. Canadian Journal Plant Science 81: 343-350.

21. Redden, R. J. and Herridge, F. 1999. Evaluation of genotypes of navy and culinary bean (Phaseolus vulgaris L.) selected for superior growth and nitrogen fixation. Australian Journal of  Experimental Agriculture 39: 975-980.

22. Vargas. L. B., Pastorina, G. N. 2001. Incompatibility may not be the rule in the Sinorhizobium fredii-soybean interaction. Soil Biology 65: 101-108.

23. Vasilas B. L and Nelson R. L. 1992. N2 fixation and dry matter accumulation in soybean lines with different seed fill periods. Canadian Journal of plant Science72:10674.