Evaluation of different strains of Rhizobium leguminosarum bv. phaseoli inoculation on ecophysiological nitrogen fixation ability of common bean, Phaseolus vulgaris L., cultivars

Subject Areas : Agroecology Journal

Mahdi Taherkhani ¹ , ghorban normohammadi ² , mohammadjavad Mirhadi ³ , hosein Haydari Sharif Abad ⁴ , amirhosein Shirani Rad ⁵

1 - Ph.D Student of Islamic Azad University of Tehran, Science and Research Branch
2 - Professor in Agronomy, Faculty of Agronomy, Islamic Azad University of Tehran, Science and Research Branch
3 - Assistant Professor in Agronomy, Faculty of Agronomy, Islamic Azad University of Tehran, Science and Research Branch
4 - Associate Professor of Seed and Plant Improvement Institute, Karaj, Iran.
5 - Assistant Professor of Seed and Plant Improvement Institute, Karaj, Iran

Received: 2008-04-03 Accepted : 2009-05-18 Published : 2009-04-21

Keywords: yield, common bean, Nitrogen fixation, Inuculant, Rhizobium leguminosarum bv. Phaseoli,

Abstract :

 In order to evaluation of ecophysiological nitrogen fixation ability of common bean (Phaseolus vulgaris L.) cultivars with some types of inoculants which contain different strains of Rhizobium leguminosarum bv. Phaseoli, a factorial experiment in randomized complete block design (RCBD) was conducted in Khoramdareh region of Iran during 2006-2007. Treatments consisted of three types of biological fertilizers including Rb117, Rb123, Rb13, a comercial inoculant (Rhizobean super plus) with two N100 (use of 100 Kg nitrogen/ha) and control (without seeds inoculation and fertilizer application) treatments and three bean cultivars of COS16 (spotted bean), Derakhshan and Akhtar (red beans). Results showed that inoculation of different bean varieties with Rhizobium strains incresead their qualititative and quantititative traits. Inoculation and nitrogen application increased yield up to 43 percent in comparison to control. Rb117 was more effective on common bean yield than other strains and caused up to 59 percent seed yield increase in comparison to non inoculant treatment. Whereas Rb123 had the least effect on yield (32%) among studied inoculants. COS16 was also the best cultivar in most traits and seed yield of this variety was 7.5 and 19 percent higher than Derakhshan and Akhtar varieties, respectively. The most protein percent were obtained from 100 kg/ha nitrogen, and Rb117 treatment. The highest and lowest protein yields were gained from Rb117 and control treatments, respectively. It was concluded that industrial biofertilizers such as Rhizobean super plus must be used as complementary materials with mineral fertilizers to achieve satisfying yield and sustainable agriculture.