Antibacterial effect of flower essential oils and plant organs’ extracts of purple coneflower on the bacterium Xanthomonas arboricola pv. juglandis

Subject Areas : Agroecology Journal

Soleiman Jamshidi ¹ , Soudabeh Andargani ² , Mehdi Oraei ³

1 - Islamic Azad University Miyaneh Branch Young Researchers and Elite Club Miyaneh, Iran
2 - M.Sc. of Horticulture Science Department Islamic Azad University Miyaneh Branch Miyaneh, Iran
3 - Assistance professor of Horticulture Science Department Islamic Azad University Miyaneh Branch Miyaneh, Iran

Received: 2014-05-02 Accepted : 2014-07-28 Published : 2014-08-23

Keywords: biocontrol, Echinacae purpurea, Antibacterial property, natural products, plant disease management, walnut blight,

Abstract :

Recently, there is an increasing attention to plant pathogen’s biocontrol considering hazards and environmental threats of chemical pesticides. Therefore, using plant materials as safe and natural antimicrobes is going to be investigated in recent years. The current study’s projective was evaluation of plant materials obtained from purple coneflower effecting on Xanthomonas arboricola pv. juglandis bacterial walnut blight agent. Aqueous, methanol, ethanol, acetone and HCl extracts of coneflower root, stem, leaf and flower were extracted by rotary set and flower essential oils using clevenger apparatus. The antimicrobial activity of coneflower extracts and essential oil was evaluated in laboratory with disc diffusion and minimal inhibitory and bactericide concentration methods. The bacterium was more sensitive to flower essential oil than extracts. Ethanol acted successfully in antimicrobial material release from plant organs. Also, aqueous extract and also HCl and acetone extracts had very limited antibacterial activities on studied bacterium. All plant extracts and essential oils were inhibitive on bacterium. Leaf extract were more inhibitive than bactericide. Regarding the results plant materials obtained from coneflower could be a remarkable potential against walnut blight bacterium and might be considered as a promising biocontrol agent in the future.

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