Effects of Dietary Organic Selenium Levels on Immune System Response and Expression of INF-γ, IL-2 and IL-6 Genes in Rats
Subject Areas : Zoology
1 - Ph.D. Graduated, Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Organic Selenium, Interferon gamma, Interleukin-2, Interleukin-6, Immune System Response, Rats,
Abstract :
High ambient temperature causes oxidative stress in animals. Selenium is a trace mineral that has an effect on the health and performance of the body in stressful conditions There are limited studies on the effect of different doses of selenium in the conditions of long-term heat stress on immune system response and the expression of genes related to inflammation. The present study was conducted to determine the effects of different doses of selenium from selenium-methionine supplement on immune system response and the expression of interferon gamma, interleukin-2 and 6 genes in rats during the hot season.25 female rats were randomly divided into five groups and five replicates. Five rats were placed in standard temperature during the experiment period and the rest of the rats were placed in heat stress. Rats in the negative control group (without heat stress) and positive control (heat stress) fed with standard pellet without additive and the other three groups fed with standard pellet plus 0.15, 0.30 and 0.45 mg selenium/kg as selenium-methionine supplement for 30 days. Adding selenium supplement to the diet increased the expression of interferon gamma gene in rats under heat stress. The relative expression of the interleukin-2 gene was lower in the groups receiving selenium supplements than in the positive control group. Also, adding selenium supplement to the diet decreased the expression of interleukin-6 gene compared to the positive control treatment. The results indicated an increase in the concentration of immunoglobulin G with the addition of selenium to the diet.
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