The effect of high intensity interval training (HIIT) on the content of mitochondrial dynamic proteins (MFN2 and FIS1) in the soleus muscle of male Wistar rats

Subject Areas : Role of Genes in Health

Mohammad Sharif Bigdeli ¹ , Neda Aghaei Bahmanbeglou ² , مژگان احمدی ³

1 - Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 - Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
3 - استادیار دانشگاه آزاد اسلامی واحد یادگار امام خمینی ( ره) شهرری - - گروه تربیت بدنی و علوم ورزشی دانشگاه ازاد اسلامی واحد یادگار امام خمینی (ره) شهرری

Received: 2024-06-23 Accepted : 2024-08-31 Published : 2025-06-21

Keywords: High-Intensity Interval Training, Mitochondrial Dynamics, Soleus Muscle,

Abstract :

Introduction: The function of mitochondria is strongly dependent on their morphological and structural changes, which is known as mitochondrial dynamics. Mitochondrial dynamics can be regulated through MFN2 and FIS1 proteins; Therefore, the aim of this research is the effect of high intensity interval training (HIIT) on the content of mitochondrial dynamic proteins (MFN2 and FIS1) in the soleus muscle of male Wistar rats. Materials and Methods: In this experimental study, 12 Wistar rats with an average weight of 280 ± 30 were randomly divided into two groups: 1. Control, 2. HIIT (6 rats in each group). The rats in the HIIT group trained for 8 weeks, 5 sessions per week, and each session consisted of 10 high-intensity intervals for 3 minutes with 2-minute rest intervals. 48 hours after the last training session, skeletal muscle tissue was harvested from the animal. The content of the variables was measured through the western blot laboratory method. Data analysis Data were analysed through independent t-test in SPSS version 27 and GraphPad Prism version 10.2.2 software. The significance level was less than p≥0.05. Results: The intracellular content of MFN2 protein did not show a significant change in the HIIT group compared to the control group (P=0.66). In contrast, the intracellular content of FIS1 protein showed a significant decrease (P=0.004). Conclusion: It seems that HIIT training cannot modulate the balance between fusion and fission of mitochondria and thus help to improve the quality and function of mitochondria.

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