The Effects of Polyethylene Terephthalate Surface Treatment by SO2 Plasma on the Polymer Hemocompatibility
Subject Areas : Journal of Environmental Friendly MaterialsF. Ahmadi 1 , A. Asef nejad 2 , M.T Khorasani 3 , M. Daliri Joopari 4
1 - Department of Biomaterial, Faculty of Nano and Advanced Material, Material and Energy Institute, Alborz, Iran.
2 - Department of Biomaterial, Faculty of Medical Sciences and Technology, Science and Research branch, Islamic Azad University, Tehran, Iran.
3 - Petrochemical Institute, Tehran, Iran
4 - Faculty of Biotechnology, National Institute of Biotechnology and Genetic Engineering,Tehran, Iran.
Keywords: Polyethylene Terephthalate, Surface Modification, SO2Plasma, Hemocompatibility,
Abstract :
Polyethylene terephthalate polymer is a member of the polyester polymer family that has high mechanical and chemical resistance. The use of artificial vessel prostheses made of polyethylene terephthalate with acceptable physical and biological characteristics is a suitable replacement for damaged vessels. The aim of this study is to investigate the effects of modifying the surface of polyethylene terephthalate with SO2 plasma on the hemocompatibility of the polymer.Polymer films were exposed to SO2 gas plasma. In order to evaluate surface chemistry changes, FTIR infrared spectroscopy test was performed. 3D imaging with atomic force microscope (AFM) was performed to examine the structural changes and MTT assay and platelet adhesion tests were carried out to investigate the changes in cell activity and coagulation.The results of infrared spectroscopy in the sample treated by plasma with SO2gas confirmed the presence of peaks related tothe symmetrical bonds of SO2in SO3 or SO4 in the sample. AFM images showed the surface structure changes. The MTT assay test proved the non-toxicity of the SO2gas plasma surface modification method. Adhesion and cell and platelet activity tests also showed the anti-clotting effect of the modified polymer.The use of plasma method with SO2gas is a suitable method to modify the surface and to increase blood compatibility of polyethylene terephthalate polymer, and probably can be used for making artificial blood vessels.
[1] He W, Benson R. Polymeric Biomaterials. Applied Plastics Engineering Handbook(Second Edition), Process., Mater., Appl., Plast. Des. Libr. 2017; 1:145-64.
[2] Chandy T, Das GS, Wilson RF, Rao GH. Use of plasma glow for surface-engineering biomolecules to enhance bloodcompatibility of Dacron and PTFE vascular prosthesis. Biomater. Res. 2000; 21(7):699-712.
[3] Ratner BD. The catastrophe revisited: blood compatibility in the 21st century. Biomater. Res. 2007; 28(34):5144-7.
[4] de Mel A, Jell G, Stevens MM, Seifalian AM. Biofunctionalization of biomaterials for accelerated in situ endothelialization: a review. Biomacromolecules. 2008; 9(11):2969-79.
[5] Holländer A, Kröpke S. Polymer Surface Treatment with SO2‐Containing Plasmas.,Plasma. Process.Polym., 2010; 7(5):390-402.
[6] Mozetič M, Primc G, Vesel A, Modic M, Junkar I, Recek N, Klanjšek-Gunde M, Guhy L, Sunkara MK, Assensio MC, Milošević S., Application of extremely non-equilibrium plasmas in the processing of nano and biomedical materials. Plasma. Sources. Sci. Technol., 2015; 24(1):015026.
[7] Hiratsuka A, Fukui H, Suzuki Y, Muguruma H, Sakairi K, Matsushima T, Maruo Y, Yokoyama K. Sulphur dioxide plasma modification on poly (methyl methacrylate) for fluidic devices. Curr. Appl. Phys. 2008; 8(2):198-205.
[8] Abbasi F, Mirzadeh H, Katbab AA. Modification of polysiloxane polymers for biomedical applications: a Rev.Polym. Int. 2001; 50:1279-87.
[9] Li YP, Li SY, Shi W, Lei MK. Hydrophobic over-recovery during aging of polyethylene modified by oxygen capacitively coupled radio frequency plasma: A new approach for stable superhydrophobic surface with high water adhesion. Surf. Coat. Technol. 2012; 206(23):4952-8.
[10]Berteau O, Mulloy B., Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology. 2003; 13(6):29R-40R.
[11] Novák I, Popelka A, Valentín M, Chodák I, Špírková M, Tóth A, Kleinová A, Sedliačik J, Lehocký M, Marônek M. Surface behavior of polyamide 6 modified by barrier plasma in oxygen and nitrogen.,Int. J. Polym. Anal. Charact. 2014; 19(1):31-8.
[12] Thompson M. Handbook of inductively coupled plasma spectrometry. 2nd Ed. Springer Science & . Business Media; 2012.
[13] Edelmann A, Diewok J, Baena JR, Lendl B. High-performance liquid chromatography with diamond ATR–FTIR detection for the determination of carbohydrates, alcohols and organic acids in red wine. Anal. Bioanal. Chem. Res. Chem. 2003; 376:92-7.
[14] Hersel U, Dahmen C, Kessler H. RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. Biomater.Res. 2003; 24(24):4385-415.
[15] Neděla O, Slepička P, Švorčík V. Surface modification of polymer substrates for biomedical applications. Mater. Today. 2017; 10(10):1115.
[16] Bartnik A, Fiedorowicz H, Jarocki R, Kostecki J, Szczurek M, Biliński A, Chernyayeva O, Sobczak JW. Physical and chemical modifications of PET surface using a laser-plasma EUV source. Appl. Phys. A. 2010; 99:831-6.
[17] Ahad I, Fiedorowicz H, Budner B, Kaldonski T, Vazquez M, Bartnik A, Brabazon D. Extreme ultraviolet surface modification of polyethylene terephthalate (PET) for surface structuring and wettability control. Acta Phys. Pol. A. 2016; 129(2):241-3.
[18] Kostov KG, Nishime TM, Castro AH, Toth A, Hein LR. Surface modification of polymeric materials by cold atmospheric plasma jet.,Appl. Surf. Sci. 2014; 314:367-75.
[19] Holländer A, Kröpke S. Polymer Surface Treatment with SO2‐Containing Plasmas. Plasma Process.Polym. 2010; 7(5):390-402.
[20] Vesel A, Recek N, Motaln H, Mozetic M. Endothelialization of polyethylene terephthalate treated in SO2 plasma determined by the degree of material cytotoxicity. Plasma. Med., 2017; 1(1):12-22.
[21] Recek N, Resnik M, Zaplotnik R, Mozetic M, Motaln H, Lah-Turnsek T, Vesel A. Cell proliferation on polyethylene terephthalatetreated in plasma created in SO2/O2 mixtures. Polym. 2017; 9(3):82.
[22] Ko TM, Lin JC, Cooper SL. Surface characterization and platelet adhesion studies of plasma-carboxylated polyethylene. J. Colloid Sciinterface. 1993; 156(1):207-17.
[23] Vesel A, Zaplotnik R, Modic M, Mozetic M. Hemocompatibility properties of a polymer surface treated in plasma containing sulfur.,Surf. Interface Anal. 2016; 48(7):6
