The effect of eight weeks of resistance training in combination with nanoliposome BCAA supplement on the expression of HSP60 and HSP70 genes in mitochondrial cardiomyocytes of heart attack model rats
Subject Areas : Role of Genes in Health
Elham Farhadfar
1
,
Makan Khajevandi
2
1 - Department of Physical Education and Sport Science, Dezful Branch, Islamic Azad University, Dezful
2 - - Department of Physical Education and sport Science, Center Tehran Branch, Islamic Azad University, Tehran, Iran
Keywords: : resistance training, BCAA nanoliposome supplement, HSP60 and HSP70 and heart attack model rats,
Abstract :
The purpose of this study was to investigate the effect of eight weeks of resistance training in combination with nanoliposome BCAA supplement on the expression of HSP60 and HSP70 genes in mitochondrial cardiomyocytes of heart attack model rats. 32 male Wistar rats with heart attack model were randomly divided into 4 groups: control, supplemented (BCAA nanoliposome), combined (resistance training + BCAA nanoliposome supplement) and training (resistance training). Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. In the supplement and combined groups, 5 days a week and for 8 weeks, BCAA nanoliposome supplement was received by gavage in the amount of 600 mg per kilogram of body weight. The relative expression of mitochondrial HSP60 and HSP70 genes was obtained using Real-time PCR method. The data were analyzed using two-way analysis of variance and Tukey's post hoc test. The results of the two-way analysis of variance test showed that the average expression of HSP60 and HSP70 genes in cardiomyocytes in the trained groups with and without BCAA consumption (exercise + supplement and exercise) was significantly higher than the untrained groups with and without consumption. BCAA (supplement and control. The results of this research clearly show the importance of HSP60 and HSP70 in the proliferation and differentiation of muscle cells along with regeneration from damage caused by heart infarction.
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2. سموات طاهره، حجت زاده علی. برنامه‌های پیشگیری و کنترل بیماری‌های قلبی-عروقی. وزارت بهداشت درمان و آموزش پزشکی. معاونت بهداشت. مرکز مدیریت بیماری‌های غیرواگیر. اداره قلب و عروق. 1390.
3. قراخانلو رضا، ملانوری مهدیه. نگاهی به سازگاری‌های سلولی و مولکولی به تمرینات ورزشی. انتشارات حتمی. 1394. چاپ اول.
4. کبودانیان سوسن، غضنفری طوبی، شاکری راحله. بیوشیمی مرگ سلولی. انتشارات دانشگاه شاهد. 1393. چاپ اول.
5. لودیش هاروی، برک آرنولد، کیسر کریس و همکاران. زیست شناسی سلولی و مولکولی. ترجمه: ستوده نیا عبدالحسین، پروانه لیلا، قربانی محمدحسین، آموزگار افسانه. انتشارات کتاب ارجمند. 1390. چاپ اول.
6. مرکز کنترل و پیشگیری از بیماری‌ها در آمریکا (www.cdc.gov/chronicdisease/overview/index.htm)
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9. Amin H, Vachris J, Hamilton A, Steuerwald N, Howden R, Arthur ST. GSK3β inhibition and LEF1 upregulation in skeletal muscle following a bout of downhill running. J Physiol Sci.2014. 64(1):1-11.
10. Javid Tabrizi N, Bashiri J, Narimani Rad M. [Effect of 12 weeks of treadmill aerobic training on cytochrome C and Caspase-9 gene expression in cardiac muscle of male rats (Persian)]. Qom Univ Med Sci J. 2017; 11(6):1-9.
11. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. J of Exer Rehabi. 2016; 9(2):219-22.
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