Phytochemical, antioxidant and anti-Alzheimer evaluation of silver nanoparticles synthesized with aqueous extract of Alhagi persarum

Subject Areas : Biochemistry

Niloofar Vahedpouri ¹ , amir arasteh ²

1 - Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2 - عضو هیئت عملی رشت، دانشگاه آزاد اسلامی واحد رشت، دانشکده ی علوم پایه، گروه میکروبیولوژی

Received: 2024-05-14 Accepted : 2024-12-06 Published : 2024-12-06

Keywords: Keywords: Alhagi persarum, antioxidant, acetylcholinesterase, Alzheimer's disease, nanoparticle ,

Abstract :

Abstract Alhagi persarum is one of the resistant plants of dry regions, which has a special place in Iranian traditional medicine. The aim of this research was to investigate the phytochemical, antioxidant and anti-Alzheimer properties of silver nanoparticles synthesized with the aqueous extract of A. persarum. The compounds in the extract were determined by GC-MS and the inhibition rate of free radicals was determined by DPPH test and the inhibition rate of acetylcholinesterase enzyme was determined by spectrometry method. Inhibition of the production of amyloid fibrils was investigated by fluorescence spectroscopy and confirmed by electron microscopy. The production of silver nanoparticles was confirmed by visible and infrared spectroscopy, and its structure was investigated by dynamic light scattering (DLS), zeta potential, scanning electron microscopy (SEM) and X-ray diffraction spectroscopy (XRD). The most compounds in the extract were Thiophen, Octadecadienoic acid and Inositol. By increasing the concentration of nanoparticles, the antioxidant activity increased and the most inhibitory effects on acetyl-cholinesterase enzyme as well as the most inhibitory effects on the production of amyloid fibrils were observed at low concentrations of nanoparticles. A. persarum plant extract, with its antioxidant and inhibitory effects on acetylcholinesterase enzyme, can be used as a suitable food supplement to reduce clinical complications in Alzheimer's patients.

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