The Effect of 12 Weeks of Broccoli Supplementation with Combined Training on Plasma Hepatokines in Men with Type 2 Diabetes

Subject Areas : Journal of Animal Biology

Fazlali Karimi ¹ (1- Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran)
حسین عابدنطنزی ² (1- Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran)
شهرام سهیلی ³ (2- Department of Physical Education and Sport Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran)
ماندانا غلامی ⁴ (1- Department of Physical Education and Sport Science, Science and Research Branch, Islamic Azad University, Tehran, Iran)

Received: 2024-05-07 Accepted : 2024-06-10 Published : 2024-08-06

Keywords: Combined training, Broccoli, Type 2 diabetes, Insulin resistance,

Abstract :

Diabetes is one of the main causes of death in developing countries, which can be improved by various factors such as physical activity and proper diet. The purpose of this study was to investigate the effect of broccoli powder consumption along with combined training on some selected hepatokines in men with type 2 diabetes. This research was semi-experimental and practical type and was pre-test and post-test. For this purpose, 44 diabetic male volunteers were randomly divided into 4 groups of 11 people including control (placebo recipient), supplement (supplement recipient), exercise + placebo groups, and exercise + supplement. The training protocol included 45 minutes of resistance training with an intensity of 60-70% of one maximum repetition, 30 minutes of aerobic exercises (running) with an intensity of 60-70% of the maximum heart rate. Broccoli (supplement) was also prepared in powder form and 10 grams per day was given to each volunteer for 12 weeks. Blood samples were taken 48 hours before the first training session and 48 hours after the last training session and were used to analyze the indicators. Intergroup analysis of data was done using a two-way analysis of variance and a correlated t-test was used for intra-group differences. A significant difference between the groups was observed in ANGPTL3 and ANGPTL4 (P<0.001). Also, the examination of changes within the group showed that in supplementary, exercise, and supplementary exercise groups, a significant decrease was observed in ANGPTL3 and ANGPTL4 (p < 0.05). The results of the present study showed that 12 weeks of combined exercise along with broccoli supplementation improves selected hepatokines and insulin resistance. This improvement was greater in the group that exercised and took supplements at the same time.

References:

Aborehab, N. M., El Bishbishy, M. H., & Waly, N. E. (2016). Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats. BMC complementary and alternative medicine, 16(1), 1-10.
Abu-Farha, M., Al-Khairi, I., Cherian, P., Chandy, B., Sriraman, D., Alhubail, A., . . . Abubaker, J. (2016). Increased ANGPTL3, 4 and ANGPTL8/betatrophin expression levels in obesity and T2D. Lipids in health and disease, 15(1), 181.
Askari, R., & Hamedinia, M. R. (2015). The effect of combined and endurance training on plasma apelin and insulin insensitivity among some overweight females. Iranian Journal of Diabetes and Metabolism, 14(5), 345-356.
Bahadoran, Z., Mirmiran, P., & Azizi, F. (2013). Potential efficacy of broccoli sprouts as a unique supplement for management of type 2 diabetes and its complications. Journal of medicinal food, 16(5), 375-382.
Bahadoran, Z., Tohidi, M., Nazeri, P., Mehran, M., Azizi, F., & Mirmiran, P. (2012). Effect of broccoli sprouts on insulin resistance in type 2 diabetic patients: a randomized double-blind clinical trial. International journal of food sciences and nutrition, 63(7), 767-771.
Bell, J. A., Kivimaki, M., & Hamer, M. (2014). Metabolically healthy obesity and risk of incident type 2 diabetes: a meta‐analysis of prospective cohort studies. Obesity reviews, 15(6), 504-515.
Blüher, M. (2014). Adipokines–removing road blocks to obesity and diabetes therapy. Molecular metabolism, 3(3), 230-240.
Burr, J. F., Rowan, C. P., Jamnik, V. K., & Riddell, M. C. (2010). The role of physical activity in type 2 diabetes prevention: physiological and practical perspectives. The Physician and sportsmedicine, 38(1), 72-82.
Christopoulou, E., Elisaf, M., & Filippatos, T. (2019). Effects of angiopoietin-like 3 on triglyceride regulation, glucose homeostasis, and diabetes. Disease markers, 2019.
Cinkajzlová, A., Mráz, M., Lacinová, Z., Kloučková, J., Kaválková, P., Kratochvílová, H., . . . Škrha, J. (2018). Angiopoietin-like protein 3 and 4 in obesity, type 2 diabetes mellitus, and malnutrition: the effect of weight reduction and realimentation. Nutrition & diabetes, 8(1), 1-11.
Colberg, S. R., Sigal, R. J., Fernhall, B., Regensteiner, J. G., Blissmer, B. J., Rubin, R. R., . . . Braun, B. (2010). Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement. Diabetes care, 33(12), e147-e167.
Dastah, S., & Babaei, S. (2021). Effect of aquatic training on serum Fetuin-A, ANGPTL4 and FGF21 levels in type 2 diabetic obese women. Journal of Applied Health Studies in Sport Physiology, 8(2), 51-60.
Evans, J. L., Goldfine, I. D., Maddux, B. A., & Grodsky, G. M. (2003). Are oxidative stress− activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes, 52(1), 1-8.
Fagiani, E., & Christofori, G. (2013). Angiopoietins in angiogenesis. Cancer letters, 328(1), 18-26.
Falcão-Pires, I., Castro-Chaves, P., Miranda-Silva, D., Lourenco, A. P., & Leite-Moreira, A. F. (2012). Physiological, pathological and potential therapeutic roles of adipokines. Drug discovery today, 17(15-16), 880-889.
gelardi, n., farzanegi, p., & abaszade, h. (2020). Exploring the effect of broccoli on some cardiovascular risk factors and insulin resistance with combined aerobic-resistance exercise training in men with type 2 diabetes. Journal of Jiroft University of Medical Sciences, 7(1), 292-301.
Hordern, M. D., Dunstan, D. W., Prins, J. B., Baker, M. K., Singh, M. A. F., & Coombes, J. S. (2012). Exercise prescription for patients with type 2 diabetes and pre-diabetes: a position statement from Exercise and Sport Science Australia. Journal of science and medicine in sport, 15(1), 25-31.
Inukai, K., Nakashima, Y., Watanabe, M., Kurihara, S., Awata, T., Katagiri, H., . . . Katayama, S. (2004). ANGPTL3 is increased in both insulin-deficient and-resistant diabetic states. Biochemical and biophysical research communications, 317(4), 1075-1079.
Kersten, S., Lichtenstein, L., Steenbergen, E., Mudde, K., Hendriks, H. F., Hesselink, M. K., . . . Müller, M. (2009). Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids. Arteriosclerosis, thrombosis, and vascular biology, 29(6), 969-974.
Köster, A., Chao, Y. B., Mosior, M., Ford, A., Gonzalez-DeWhitt, P. A., Hale, J. E., . . . Yang, D. D. (2005). Transgenic angiopoietin-like (angptl) 4 overexpression and targeted disruption of angptl4 and angptl3: regulation of triglyceride metabolism. Endocrinology, 146(11), 4943-4950.
Kusminski, C. M., Bickel, P. E., & Scherer, P. E. (2016). Targeting adipose tissue in the treatment of obesity-associated diabetes. Nature reviews Drug discovery, 15(9), 639.
Li, G., Zhang, H., & Ryan, A. S. (2020). Skeletal muscle angiopoietin-like protein 4 and glucose metabolism in older adults after exercise and weight loss. Metabolites, 10(9), 354.
Maritim, A., Sanders, a., & Watkins Iii, J. (2003). Diabetes, oxidative stress, and antioxidants: a review. Journal of biochemical and molecular toxicology, 17(1), 24-38.
Mattijssen, F., & Kersten, S. (2012). Regulation of triglyceride metabolism by Angiopoietin-like proteins. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1821(5), 782-789.
McCulloch, L. J., Bramwell, L. R., Knight, B., & Kos, K. (2020). Circulating and tissue specific transcription of angiopoietin-like protein 4 in human Type 2 diabetes. Metabolism, 154192.
Mirmiran, P., Mohtadinia, J., Hedayati, M., & Shakeri, N. (2011). Effects of broccoli sprout powder on fasting serum glucose and lipid profiles in type 2 diabetic patients. Iranian Journal of Endocrinology and Metabolism, 13(1), 18-25.
Oike, Y., Yasunaga, K., & Suda, T. (2004). Angiopoietin-related/angiopoietin-like proteins regulate angiogenesis. International journal of hematology, 80(1), 21-28.
Praet, S. F., & Van Loon, L. J. (2007). Optimizing the therapeutic benefits of exercise in type 2 diabetes. Journal of applied physiology, 103(4), 1113-1120.
Robciuc, M. R., Maranghi, M., Lahikainen, A., Rader, D., Bensadoun, A., Öörni, K., . . . Ceci, F. (2013). Angptl3 deficiency is associated with increased insulin sensitivity, lipoprotein lipase activity, and decreased serum free fatty acids. Arteriosclerosis, thrombosis, and vascular biology, 33(7), 1706-1713.
Sadeghi, A., Gholami, M., Matinhomaee, H., Aabednatanzi, H., & Ghazalian, F. (2022). Changes in the serum levels of ANGPTL3, ANGPTL4 and CRP following combined training alone or in combination with thyme ingestion in the obese men. Daneshvar Medicine, 30(2), 61-73.
Saeidi, A., Soltani, M., Daraei, A., Nohbaradar, H., Haghighi, M. M., Khosravi, N., . . . Vandusseldorp, T. A. (2021). the effects of aerobic-resistance training and broccoli supplementation on plasma dectin-1 and insulin resistance in males with type 2 diabetes. Nutrients, 13(9), 3144.
Stefan, N., & Häring, H.-U. (2013). The role of hepatokines in metabolism. Nature Reviews Endocrinology, 9(3), 144-152.
Tjeerdema, N., Georgiadi, A., Jonker, J. T., van Glabbeek, M., Dehnavi, R. A., Tamsma, J. T., . . . Rensen, P. C. (2014). Inflammation increases plasma angiopoietin-like protein 4 in patients with the metabolic syndrome and type 2 diabetes. BMJ Open Diabetes Research and Care, 2(1).
Yoshida, K., Shimizugawa, T., Ono, M., & Furukawa, H. (2002). Angiopoietin-like protein 4 is a potent hyperlipidemia-inducing factor in mice and inhibitor of lipoprotein lipase. Journal of lipid research, 43(11), 1770-1772.
Zanuso, S., Jimenez, A., Pugliese, G., Corigliano, G., & Balducci, S. (2010). Exercise for the management of type 2 diabetes: a review of the evidence. Acta diabetologica, 47(1), 15-22.
Zhang, C. C., Kaba, M., Ge, G., Xie, K., Tong, W., Hug, C., & Lodish, H. F. (2006). Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells. Nature medicine, 12(2), 240-245.