Intense Endurance Running Training and Supplementation with the Aqueous Extract of Ajwain Seed: Effect on the Levels of Zinc and Some zinc Transporters in the Liver Tissue of Male Wistar Rats

Subject Areas : Journal of Animal Biology

ABBAS Ghanbari Niaki ¹ (Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Mazandaran, Babolsar, Iran)
Araz Nazari ² (Faculty member of Higher educational complex of Saravan, Saravan, Iran)
Khadije Nasiri ³ (Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Mazandaran, Babolsar, Iran)

Received: 2024-04-01 Accepted : 2024-05-16 Published : 2024-08-06

Keywords: High intensity exercise, Supplementation, aqueous extract of Ajwain seed, Slc30a (ZnT), Slc39a (Zip),

Abstract :

Zinc, an essential trace element, plays a crucial role in cellular metabolism. By interacting with numerous proteins, it is essential for a wide range of biological processes, including normal growth, reproduction, DNA synthesis, cell division, gene expression, cell signaling, wound healing, bone formation, and immune system enhancement. The liver plays a central role in zinc metabolism and homeostasis in the body. Zinc concentrations in cells are tightly regulated by two families of zinc transporter proteins, Slc30a (Znt) and Slc39a (Zip), which are known to decrease and increase cytosolic zinc concentration, respectively. Forty male Wistar rats (4-5 weeks old, 189 ± 7.8 g) were randomly assigned to four groups: saline-control (SC), saline-exercise (ST), Ajwain-control (AC), and Ajwain-exercise (AT). Mice in the training group ran on a treadmill at 32 m/min for 60 minutes per session, 5 days a week, for 8 weeks. Mice were received orally (2 g in 10 ml water/kg body weight) and saline groups were treated in the same way. Data analysis was performed using SPSS version 27 software with a two-way ANOVA and Tukey's post hoc test. Ajwain supplementation significantly increased Znt5 and Zip8 gene expression compared to saline groups. Zinc levels and Znt6 and Zip7 gene expression did not show significant changes. Simultaneous implementation of exercise and supplementation with aqueous extract of fennel seeds may modulate zinc levels as well as the gene expression of some zinc transporters in the liver. These findings may provide novel insights into the underlying mechanisms of exercise and nutrition effects on liver tissue.

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