The effect of ultrasonic waves on the germination of medicinal plants seeds of Melissa officinalis, Cuminum cyminum and Plantago major
Subject Areas :Masomeh Asadi-Gakieh 1 , Mahvash Ghazvineh 2 , نسرین حاتمی قره قوینی 3 , Ali Babaei-Ghaghelestany 4
1 - Expert of Education and Renovation, Transformation held office, Agricultural-Jahad Organization of Alborz province
2 - Agronomy Expert in Agricultural-Jahad Organization of Alborz province.
3 - کارشناس ارشد اصلاح نباتات، دانشگاه آزاد اسلامی واحد کرمانشاه
4 - Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil.
Keywords: Germination Percentage, Medicinal Plants, Seed, Ultrasonic Waves,
Abstract :
This study investigated the impact of ultrasonic waves on the germination of medicinal plants, including Lemon balm (Melissa officinalis), Cumin (Cuminum cyminum), and Plantago (Plantago major). Ultrasonic treatments comprised the following durations: control (without ultrasonic waves), 5, 10, 15, and 20 minutes. The experiment, conducted in 3 replications, followed a completely randomized design in the year 2019. The results revealed a significant effect of ultrasonic waves on the germination percentage of Melissa officinalis, with a notable increase of 22% in germination under the 20-minute ultrasonic treatment compared to the control. The highest germination percentage for Cuminum cyminum seeds was obtained with 20 and 15 minutes of ultrasonic treatment, while the lowest germination percentage was observed in the control group (without ultrasonic waves). Furthermore, the results indicated that in the 15 and 20-minute ultrasonic treatments, the germination percentage of Cuminum cyminum increased by 48.90% and 47%, respectively, compared to the control. The highest germination percentage for Plantago major seeds was achieved in the 15 and 20-minute ultrasonic treatments. The lowest germination percentage was observed in the control group. In the 20-minute ultrasonic treatment, the germination percentage of Plantago major seeds increased by 19.83% compared to the control. Overall, the findings of this research demonstrated a significant increase in the germination of Melissa officinalis, Cuminum cyminum, and Plantago major seeds under the influence of ultrasonic waves compared to the control.
Addoun, N., Boual, Z., Delattre, C., Ursu, A. V., Desbrières, J., Le Cerf, D., Gardarin, C., Hentati, F., El-Hadj, M. D. O., Michaud, P., & Pierre, G. (2020). Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf. International Journal of Biological Macromolecules, 155, 1333–1341. https://doi.org/10.1016/j.ijbiomac.2019.11.106
Akbar, S. (2020). Handbook of 200 Medicinal Plants: A Comprehensive Review of Their Traditional Medical Uses and Scientific Justifications. Springer Nature.
Aliero, B. (2004). Effects of sulphuric acid, mechanical scarification and wet heat treatments on germination of seeds of African locust bean tree, Parkia biglobosa. African Journal of Biotechnology, 3(3), 179–181.
Archangi, A., Heidari, B., & Mohammadi-Nejad, G. (2019). Association between seed yield-related traits and cDNA-AFLP markers in cumin (Cuminum cyminum) under drought and irrigation regimes. Industrial Crops and Products, 133, 276–283. https://doi.org/10.1016/j.indcrop.2019.03.038
Chiu, K. Y., & Sung, J. M. (2014). Use of ultrasonication to enhance pea seed germination and microbial quality of pea sprouts. International Journal of Food Science & Technology, 49(7), 1699–1706.
Cravotto, G., Boffa, L., Mantegna, S., Perego, P., Avogadro, M., & Cintas, P. (2008). Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves. Ultrasonics Sonochemistry, 15(5), 898–902.
de Carvalho Silvello, M. A., Martínez, J., & Goldbeck, R. (2019). Increase of reducing sugars release by enzymatic hydrolysis of sugarcane bagasse intensified by ultrasonic treatment. Biomass and Bioenergy, 122, 481–489. https://doi.org/10.1016/j.biombioe.2019.01.032
Gallo, M., Ferrara, L., & Naviglio, D. (2018). Application of Ultrasound in Food Science and Technology: A Perspective. Foods, 7(10), Article 10. https://doi.org/10.3390/foods7100164
Goussous, S., Samarah, N., Alqudah, A., & Othman, M. (2010). Enhancing seed germination of four crop species using an ultrasonic technique. Experimental Agriculture, 46(2), 231–242.
Kalita, D., Sarma, B., & Srivastava, B. (2017). Influence of germination conditions on malting potential of low and normal amylose paddy and changes in enzymatic activity and physico chemical properties. Food Chemistry, 220, 67–75. https://doi.org/10.1016/j.foodchem.2016.09.193
Lee, Y.-I., Lee, N., Yeung, E. C., & Chung, M.-C. (2005). Embryo Development of Cypripedium formosanum in Relation to Seed Germination In Vitro. Journal of the American Society for Horticultural Science, 130(5), 747–753. https://doi.org/10.21273/JASHS.130.5.747
Li, W., Ma, H., He, R., Ren, X., & Zhou, C. (2021). Prospects and application of ultrasound and magnetic fields in the fermentation of rare edible fungi. Ultrasonics Sonochemistry, 76, 105613. https://doi.org/10.1016/j.ultsonch.2021.105613
Liu, J., Wang, Q., Karagić, \DJura, Liu, X. V., Cui, J., Gui, J., Gu, M., & Gao, W. (2016). Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye. Scientific Reports, 6(1), 22403.
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118.
Machikowa, T., Kulrattanarak, T., & Wonprasaid, S. (2013). Effects of ultrasonic treatment on germination of synthetic sunflower seeds. International Journal of Agricultural and Biosystems Engineering, 7(1), 1–3.
Miraj, S., Rafieian-Kopaei, & Kiani, S. (2017). Melissa officinalis L: A Review study with an antioxidant prospective. Journal of Evidence-Based Complementary & Alternative Medicine, 22(3), 385–394.
Miyoshi, K., & Mii, M. (1988). Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor, in asymbiotic culture. Scientia Horticulturae, 35(1–2), 127–130.
Naumenko, N., Potoroko, I., & Kalinina, I. (2022). Stimulation of antioxidant activity and γ-aminobutyric acid synthesis in germinated wheat grain Triticum aestivum L. by ultrasound: Increasing the nutritional value of the product. Ultrasonics Sonochemistry, 86, 106000.
Nazari, M., Sharififar, A., & Asghari, H. R. (2014). Medicago scutellata seed dormancy breaking by ultrasonic waves. Plant Breeding and Seed Science, 69(1), 15.
Omidbaigi, R. (2005). Production and processing of medicinal plants. Astan’eQods’ eRazavi Publication, 3.
Sharififar, A., Nazari, M., & Asghari, H. R. (2015). Effect of ultrasonic waves on seed germination of Atriplex lentiformis, Cuminum cyminum, and Zygophyllum eurypterum. Journal of Applied Research on Medicinal and Aromatic Plants, 2(3), 102–104. https://doi.org/10.1016/j.jarmap.2015.05.003
Shashikanthalu, S. P., Ramireddy, L., & Radhakrishnan, M. (2020). Stimulation of the germination and seedling growth of Cuminum cyminum L. seeds by cold plasma. Journal of Applied Research on Medicinal and Aromatic Plants, 18, 100259.
Soltani, E., Mortazavian, S. M. M., Faghihi, S., & Akbari, G. A. (2019). Non-deep simple morphophysiological dormancy in seeds of Cuminum cyminum L. Journal of Applied Research on Medicinal and Aromatic Plants, 15, 100222. https://doi.org/10.1016/j.jarmap.2019.100222
Toth, I. (2012). The effects of ultrasound exposure on the germination capacity of birdsfoot trefoil (Lotus corniculatus L.) seeds. Roman J Biophys, 22(1), 13–20.
Verma, P. S., Singh, A., Rahaman, L., & Bahl, J. (2015). Lemon balm (Melissa officinalis L.) an herbal medicinal plant with broad therapeutic uses and cultivation practices: A review. Internal Journal of Recent Advances in Multidisciplinary Research, 2, 928–933.
Wang QuanZhen, W. Q., Chen Guo, C. G., Yersaiyiti, H., Liu Yuan, L. Y., Cui Jian, C. J., Wu ChunHui, W. C., Zhang YunWei, Z. Y., & He XueQing, H. X. (2012). Modeling analysis on germination and seedling growth using ultrasound seed pretreatment in switchgrass. https://www.cabidigitallibrary.org/doi/full/10.5555/20123401016
Yaldagard, M., Mortazavi, S. A., & Tabatabaie, F. (2008). Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. African Journal of Biotechnology, 7(14). https://www.ajol.info/index.php/ajb/article/view/59035
Yang, H., Gao, J., Yang, A., & Chen, H. (2015). The ultrasound-treated soybean seeds improve edibility and nutritional quality of soybean sprouts. Food Research International, 77, 704–710. https://doi.org/10.1016/j.foodres.2015.01.011
Yu, M., Liu, H., Shi, A., Liu, L., & Wang, Q. (2016). Preparation of resveratrol-enriched and poor allergic protein peanut sprout from ultrasound treated peanut seeds. Ultrasonics Sonochemistry, 28, 334–340.
