Studies on seminal plasma enzymes (GOT, GPT and LDH) profle and its relationship with quality of buffalo frozen semen

Subject Areas : clinical veterinary science

عبدالرضا  Rastegarnia. ¹ (Department of Clinical Sciences، Faculty of Veterinary Medicine، Islamic Azad University Urmia، Branch، Urmia – Iran ,.P.O.BOX 969)
یحیی  Eviaz Sahara. ² (Graduated from the Faculty of Veterinary Medicine، Islamic Azad University Urmia branch، Urmia - Iran)
وحید  Shafepour, ³ (Buffalo Breeding and Training Center, Jabal-Urmia, Urmia-,Iran)

Received: 2016-11-02 Accepted : 2016-11-02 Published : 2010-06-22

Keywords: Buffalo, Frozen semen, GOT , GPT, LDH enzymes,

Abstract :

This study was conducted to evaluate seminal enzyme (GOT, GPT and LDH) profle and its relationship withquality of buffalo frozen spermatozoa. For this purpose ,twenty-fve split pooled ejaculates from fve buffalo bulbsin Buffalo Breeding and Training center (Jabal-Urmia) possessing more than 80% visual sperm motility , werediluted at 37◦C in tris- egg yolk extender. The diluted semen was cooled to 4◦C within 2 hours, equilibrated at 4◦Cfor 4-6 hours following the addition of glycerol , flled in 0.5 ml French straws and were subjected to coolingcondition before being plunged into liquid nitrogen . Semen was thawed at 37◦C for 40 seconds after 72 hours ofstorage inside liquid nitrogen. Post thaw visual sperm motility, plasma membrane integrity and acrosome morphology of each frozen semen sample were assessed by warm plate microscopy at 37◦C, hypo osmotic swellingtest, and Giemsa staining, respectively. LDH and GOT(AST) ،GPT(ALT) enzyme activities were measured usingkinetic oxidation of lactate to private method and colorimetric method , respectively. Overall mean activity offrozen semen GOT (AST) ،GPT (ALT) and LDH enzyme activities were 140.4±18.5 (IU/L), 270.4±58.5 (IU/L)and 90.4±32.7 (IU/L) ,respectively. The relationship between the activities of these enzymes and semen qualityshowed only a positive correlation between increased LDH and GOT (AST) ،GPT (ALT) enzyme activities anddecrease post thaw spermatozoa characteristics. These result revealed that seminal enzyme (GOT, GPT and LDH)profle in extender is suitable and recommendable for evaluation of buffalo frozen semen.