Molecular analysis and reduction of mycotoxin deoxynivalenol to 3-acetyl form in 20 durum wheat cultivars of Khuzestan through PCR technique and determination of AYT1 resistance gene
Subject Areas :Seyed Saeed Noorinia 1 , neda baghestani 2
1 - Masters degree from Imam Khomeini University of Boroujerd
2 - Bachelor's degree, Research and Natural Resources Center of Khuzestan Province
Keywords: PCR, SSR markers, Fusarium blight of bread wheat, mycotoxin deoxynivalenol,
Abstract :
Abstract:This study was conducted to evaluate the response of 20 durum wheat cultivars native to Khuzestan, available in the collection of the grain research department of the Seed and Plant Breeding Research Institute, to Fusarium blight during the two cropping years 2016-2017 and 2018-2019 in field conditions in two regions. Different, Khuzestan, with two climatic conditions, was implemented. The studied traits included infected clusters, spikelet infestation, seed infestation, cluster density, plant height and biological yield. Cluster analysis was used to measure and determine genetic distances and pattern diversity in wheat Fusarium resistance components of wheat. Evaluation of experimental masses using sporulation method in the field showed that most of the studied masses have a good resistance to the disease in terms of the mean disease index. The cause of resistance seems to have a genetic basis and is related to the presence of type I resistance in them. Twenty durum wheat cultivars can be used as disease-resistant parents in crosses related to wheat breeding programs. The results showed that AYT1 gene is expressed as resistant gene in wheat cultivars. Using PCR technique and SSR molecular markers in this study in order to amplify a single copy or small copies of a DNA fragment with a specific sequence of thousands or millions of copies, to achieve the genetic nature of cultivars, this method can be used as a method. Introduced for the multiplication of resistant genes in this study.
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