Effect of growth regulators on callus induction in Quince (Cydonia oblonga Mill.)
Subject Areas :elham faezi 1 , Mansoor omidi 2 , parisa abdollahi 3
1 - College of Agriculture, Science and Research Branch of Tehran
2 - college of Agricultural and Natural Resource, University of Tehran, Karaj,
3 - College of Agriculture,Tehran Science and Research branch, Islamic Azad University
Keywords: Growth regulators, Medicinal plant, quince, Meristem,
Abstract :
The plant is quince known as a medicinal plant. In vitro and induction of callus from different species of this plant are important and sensitive stages. In the present study, the ability of callusing and direct regeneration in callus medium in explants and various growth regulators were evaluated. From different parts of leaf, stems, meristem, leaf tail and callus were prepared Callus buds on the stem quince. For making callus formation used the MS medium containing three levels of 1, 2 and 4 mg / L of 2,4-D hormone and 0.1, 0.2 and 1 mg / L BAP hormone, each of growth regulators was studied as a factorial experiment in a completely randomized design with three replications on MS medium. The results of the comparison showed that the highest amount of calluses in the first culture medium belonged to the cultivar of callus buds (on woody stem) at 4 mg / L 2,4-D concentration was 19.5 mm and at 1 mg / L BAP concentration was 27.5 mm in the callus bud (on a wooden stem) Also, the highest amount of calluses in the second order was attributed to the meristem extract at 0.1 mg / L BAP concentration was 32 mm and at 1 mg / L 2,4-D concentration was 23mm belonging to the callus bud (on the Wooden stem). Direct regeneration was observed in meristem, stem and leaf.
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