The Protective Effect of Tribulus terrestris Hydroalcoholic Extract on Cisplatin-induced Cytotoxicity on Spermatogenesis on BALB/c Mice

Subject Areas : Journal of Animal Biology

Z.  Keshtmand ¹ (Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
Sh.  Oryan ² (Department of Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran)
A.  Ghanbari ³ (Fertility and Infertility Research center, Kermanshah University of Medical Sciences, Kermanshah, Iran)

Received: 2017-06-17 Accepted : 2017-06-17 Published : 2017-05-22

Keywords: Mice, Spermatogenesis, Cisplatin, Tribulus terrestris,

Abstract :

Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin use is cytotoxicity and decrease in genital gland function, azoospermia, and oligospermia. Tribulus terrestris has been used as an aphrodisiac. The present study amid to investigatethe protective effect of T. terrestris hydroalcoholic extract on cisplatin-induced cytotoxicity on spermatogenesis in mice. In this experimental study, thirty male adult mice weighning 25-30 gr were randomly divided into five groups of six members each. Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group, that received different doses of hydroalcoholic extract of T.terrestris 100-300 and 500 (mg/kg/i.p) with cisplatin respectively. The day after the last injection, weights of body and testis were measured and after anesthesia, their testes were taken out and tissue dissections were assessed. Data analysis was performed using SPSS and one-way ANOVA. Cisplatin led to a reduction significantly in the weights of body, testis (P<0.05) and spermatogenesis compared to the control group (P<0.001), while in treated groups with T. terrestris, the weights of body, testis, and spermatogenesis were higher compared with cisplatin group. Extract of T. terrestris ­could effect ­of protective on cisplatin-induced cytotoxicity on sperm morphology related to the presence of antioxidant components.

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