Investigation of the Effects of Harmine Alkaloid on Bovine Oocyte in Vitro Maturation

Subject Areas : Journal of Animal Biology

Z. Hemmati ¹ , M. Heydari Nasrabadi ² , P. Khodarahmi ³

1 - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
2 - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
3 - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran

Received: 2018-04-21 Accepted : 2018-12-06 Published : 2018-11-22

Keywords: In vitro maturation, Alkaloids harmine, Bovine oocyte,

Abstract :

In vitro maturation of oocytes is the technique that can reduce costs and eliminate the side effects of the use of gonadotropins for in vitro fertilization. Conducting research on the development and improvement of in vitro culture conditions for human oocyte maturation is very difficuly. The use domestic animals has provided the most advanced and best system to study the maturation of immature follicles in the laboratory. Due to the active ingredient in the harmine alkaloid with multiple pharmacological effects including anti-free radical, anti-inflammation and immune system, etc., the present study assesses the effects of harmine alkaloid on bovine oocyte maturation. In this study, at least three layers of cumulus oocytes aspirated from 2-8 mm follicles obtained from bovine ovaries from slaughterhouses after three washes in the washing environment were incubated for 22-24 hours in maturation environments with different concentrations of 0.5, 1, 2.5, and 5 µg/ml of the alkaloid harmine at a temperature of 38.5 ° C with 5% carbon dioxide. After a while, the cumulus incubation was removed from surrounding eggs were removed and the release of polar corpuscle (maturity) was assessed. Results were evaluated using SPSS and ANOVA. Research results showed the positive impact of the alkaloid harmine at 1 µg/ml (p <0.05) such that the maturity of the groups treated with doses of 0.5, 1, 2.5 and 5 micrograms per milliliter were 59.85, 72.65, 64.34, and 37.22 percent, respectively, which was 56.93 percent in the control group. Adding 1 µg/ml of alkaloids harmine led to the maturity environment increased the maturation of the bovine oocytes, which can be used as a supplement in oocyte maturation environments.

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