The Effect of Nitro-chalcone Substance on Breast Cancer Induced in BALB/c Mice

Subject Areas : Journal of Animal Biology

Fatemeh Sabri ¹ , Parichehreh Yaghmaei ² , Azadeh Ebrahim Habibi ³ , Shiva Irani ⁴

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Endocrinology and Metabolism Research Institute of Tehran University of Medical Sciences, Tehran, Iran
4 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2018-02-19 Accepted : 2018-02-19 Published : 2017-11-22

Keywords: breast cancer, BALB/c, Nitrochalcone,

Abstract :

Cancer is an uncontrollable cell growth. Breast cancer is a kind of cancers that itr main approach of treatment is chemotherapy with side effects. Today, the use of natural ingredients, such as chalcones, is used to treat cancer. We aimed to induce apoptosis with nitrochalcone drugs in breast cancer cells of 4 t1 and on BALB/c mice. Drug toxicity was measured by MTT assay at 24 and 48 h and at concentrations of 20, 30, 40, 50 μM. Hematoxylin-azine was evaluated after treatment of tumor mice. Then, significant results were analyzed using SPSS and ANOVA. The results of MTT assay showed nitrocellulose 50% drug toxicity at 24 h at all concentrations. Apoptosis was also observed in the histological examination. Therefore, nitrochalcone drugs can be a good way to treat breast cancer.

References:

1. کاویانی.، ا و همکاران. 1389. راهنمای جامع بیماری­های پستان، مرکز تحقیقات سرطان پستان جهاد دانشگاهی، ص: 50-1


2. Abeloff M.D., Armitage J.O., Niederhuber J.E., Kastan M.B., McKenna W.G., 2004. Review of Clinical Oncology. Saunders, 2224 pp.


3. Aggarwal B.B., Vijayalekshmi R.V., Sung, B., 2009. Targeting inflammatory pathways for prevention and therapy of cancer: short-term friend, long-term foe. Clinical Cancer Research, 15(2): 425-430.


4. Aljarrah K., Mhaidat N.M., Al-Akhras M.A.H., Aldaher A.N., Albiss B.A., Aledealat K., Alsheyab F.M., 2012. Magnetic nanoparticles sensitize MCF-7 breast cancer cells to doxorubicin-induced apoptosis. World Journal of Surgical Oncology, 10(1): 62.


5. Cho K., Wang X.U., Nie S., Shin D.M., 2008. Therapeutic nanoparticles for drug delivery in cancer. Clinical Cancer Research, 14(5): 1310-1316.


6. Ferreira J.F., 2009. Artemisia species in small ruminant production: their potential antioxidant and anthelmintic effects. In Appalachian Workshop and Research Update: Improving small ruminant grazing practices; Morales, M., Ed.; Mountain State University/USDA: Beaver, USA, pp: 53-70.


7. George T.W., Niwat C., Waroonphan S., Gordon M.H., Lovegrove J.A., Paterson E., 2007. Effects of chronic and acute fruit and vegetable juice consumption on cardiovascular disease risk factors. In II International Symposium on Human Health Effects of Fruits and Vegetables-FAVHEALTH, 841: 201-206.


8. Jardim G.A., Guimarães T.T., Maria do Carmo F.R., Cavalcanti B.C., de Farias K.M., Pessoa C., Gatto C.C., Nair, D.K., Namboothiri I.N., da Silva Júnior E.N., 2015. Naphthoquinone-based chalcone hybrids and derivatives: synthesis and potent activity against cancer cell lines. Medicinal Chemistry Communication s, 6(1): 120-130.


9. Li J.J., Gribble G. eds., 2006. Palladium in heterocyclic chemistry: a guide for the synthetic chemist (Vol. 26). Elsevier Science, 658 pp.


10.  Mokhtari M.J., Motamed N., Shokrgozar M.A., 2008. Evaluation of silibinin on the viability, migration and adhesion of the human prostate adenocarcinoma (PC-3) cell line. Cell Biology International, 32(8): 888-892.


11.  Mosmann T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2): 55-63.


12.  Mousavi S.M., Montazeri A., Mohagheghi M.A., Jarrahi A.M., Harirchi I., Najafi M., Ebrahimi M., 2007. Breast cancer in Iran: an epidemiological review. The Breast Journal, 13(4): 383-391.


13.  Pegram M.D., Pienkowski T., Northfelt D.W., Eiermann W., Patel R., Fumoleau P., Quan E., Crown J., Toppmeyer D., Smylie M., Riva, A., 2004. Results of two open-labels, multicenter phase II studies of docetaxel, platinum salts, and trastuzumab in HER2-positive advanced breast cancer. Journal of the National Cancer Institute, 96(10): 759-769.


14. Sasayama T., Tanaka K., Mizukawa K., Kawamura A., Kondoh T., Hosoda K., Kohmura, E., 2007. Trans-4-lodo, 4′-boranyl-chalcone induces antitumor activity against malignant glioma cell lines in vitro and in vivo. Journal of Neuro-Oncology, 85(2): 123-132.


15.  Singh S., Sharma P.K., Kumar N., Dudhe R., 2011. A Review on a Versatile Molecule: Chalcone. Asian Journal of Pharmaceutical and Biological Research (AJPBR), 1(3): 412- 418.


16.  www.atcc.org.


17. www.lookchem.com/4-NITROCHAL CONE/