The Comparison of the Influence of 12-Week High- Intensity Interval Training and Continuous Moderate Intensity Training on PGC-1α and Tfam Mitochondrial Proteins Expressions in Gastrocnemius Muscle of Elderly Rats

Subject Areas : Journal of Animal Biology

A. Bakhtiyari ¹ , A. Gaeni ² , S. Choobineh ³ , M.R. Kordi ⁴ , M. Hedayati ⁵

1 - Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2 - Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 - Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
4 - Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
5 - Prevention and Treatment of Obesity Research center, Research Institute for Endocrine Science, Shahid Beheshti University of Medical Sciences and health services, Tehran, Iran

Received: 2019-05-10 Accepted : 2019-09-03 Published : 2019-09-21

Keywords: Aging, Mitochondrial biogenesis, High-intensity interval training, Moderate continuous training,

Abstract :

Endurance training increase the proteins, enzymes and mitochondrial functions in human and animal samples. The purpose of this study was comparison of the influence of 12-week high-intensity interval training (HIIT) and moderate intensity continuous training (MICT) on PGC-1a and Tfam mitochondrial proteins expression in gastrocnemius muscle of elderly rats. In this study 45 elderly 22 months old Wistar rats (weighing 325-375g) were randomly divided into three groups: HIIT, MICT, and the control group. The exercise in the HIIT group was started with 20 minutes (2 minutes at 85-90% VO2max intensity and 2 minutes of recovery period at 45-50% VO2max intensity) in the first week and then reached to 48 minutes in the 12th week. The training program in the MICT group was begun with 20 minutes (at 65-70% VO2max intensity) and reached to 48 minutes in the 12th week. The expression levels of PGC-1α and Tfam proteins in gastrocnemius muscles were measured by Western blot method. One-way ANOVA was used to analyze the data. The PGC-1α expression was significantly increased in the HIIT (P< 0.001) and MICT (P<0.01) groups compared to the control group. Also, PGC-1α levels were significantly increased in the HIIT group compared to the MICT group (P<0.05). Tfam expression was significantly increased in the HIIT (P<0.01) and MICT (P<0.05) groups compared to the control group. HIIT resulted in a greater increase in PGC-1α and Tfam expressions in the gastrocnemius muscle of elderly rats compared to MICT.

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