The Comparison of the Influence of 12-Week High- Intensity Interval Training and Continuous Moderate Intensity Training on PGC-1α and Tfam Mitochondrial Proteins Expressions in Gastrocnemius Muscle of Elderly Rats

Subject Areas : Journal of Animal Biology

A.  Bakhtiyari ¹ (Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran)
A.  Gaeni ² (Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran)
S.  Choobineh ³ (Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran)
M.R.  Kordi ⁴ (Department of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran)
M.  Hedayati ⁵ (Prevention and Treatment of Obesity Research center, Research Institute for Endocrine Science, Shahid Beheshti University of Medical Sciences and health services, Tehran, Iran)

Received: 2019-05-10 Accepted : 2019-09-03 Published : 2019-09-21

Keywords: Aging, Mitochondrial biogenesis, High-intensity interval training, Moderate continuous training,

Abstract :

Endurance training increase the proteins, enzymes and mitochondrial functions in human and animal samples. The purpose of this study was comparison of the influence of 12-week high-intensity interval training (HIIT) and moderate intensity continuous training (MICT) on PGC-1a and Tfam mitochondrial proteins expression in gastrocnemius muscle of elderly rats. In this study 45 elderly 22 months old Wistar rats (weighing 325-375g) were randomly divided into three groups: HIIT, MICT, and the control group. The exercise in the HIIT group was started with 20 minutes (2 minutes at 85-90% VO2max intensity and 2 minutes of recovery period at 45-50% VO2max intensity) in the first week and then reached to 48 minutes in the 12th week. The training program in the MICT group was begun with 20 minutes (at 65-70% VO2max intensity) and reached to 48 minutes in the 12th week. The expression levels of PGC-1α and Tfam proteins in gastrocnemius muscles were measured by Western blot method. One-way ANOVA was used to analyze the data. The PGC-1α expression was significantly increased in the HIIT (P< 0.001) and MICT (P<0.01) groups compared to the control group. Also, PGC-1α levels were significantly increased in the HIIT group compared to the MICT group (P<0.05). Tfam expression was significantly increased in the HIIT (P<0.01) and MICT (P<0.05) groups compared to the control group. HIIT resulted in a greater increase in PGC-1α and Tfam expressions in the gastrocnemius muscle of elderly rats compared to MICT.

References:

1.  Bækkerud F.H., Solberg F., Leinan I.M., Wislff U., Karlsen T., 2016.         Comparison of three popular exercise modalities on VO2max in overweight and obese Medicine and Science in Sports and Exercise, 100(48): 491-498.


2. Bai P., Canto C., Oudart H., Brunyanszki A., Cen Y.N., Thomas C., Yamamoto A., Kiss B., Houtkooper R.H., Schoonjans K., Schreiber V., Sauve A.A., 2011. PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation. Cell Metabulism, 20(4): 461-468.


3. Billat L.V., 2001. Interval training for performance: a scientific and empirical practice. Sports Medicine, 40(31): 13-31.


4. Brenmoehl J., Hoeflich A., 2013. Dual control of mitochondrial biogenesis by sirtuin 1 and sirtuin 3. Mitochondrion, 13(6): 755-761.


5. Chounghun K., Eunhee C., 2010. Exercise training attenuates aging-associated mitochondrial dysfunction in rat skeletal muscle: Role of PGC-1α. Archives of Biochemistry and Biophysics, 501(12):  79-90.


6. Dae Yun Seo., Sung Ryul Lee., Nari Kim., Kyung S.K. 2016. Age-related changes in skeletal muscle
   mitochondria: the role of exercise., Integral Medical Research, 30(5): 182-189.  


7. Erika K., Zsofia S., Mustafa A., Istvan B., Hisashi N., Sataro G., 2017. Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats. The Journals of Gerontology, 40(22): 76-83.


8. Granata C., Oliveira R., 2016. Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle. FASEB Journal, 40(6): 100-125.


9. Hatle H., Stobakk P.K., Molmen H.E., Bronstad E., Tjonna A.E., Steinshamn S., 2014. Effect of 24 sessions of high-intensity aerobic interval training carried out at either high or moderate frequency, a randomized trial. PLoS One, 9, 88-75.


10.  Holloszy J.O., Coyle E.F., 1984. Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology, 120(56): 831-838.


11.  Hoydal M.A., Wisloff U., Kemi O.J., Ellingsen O., 2007. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention and Rehabilitation, 14(6): 753-60.


12.  Johnson ML., Irving B.A., Lanza I.R., Vendelbo M.H., Konopka A.R., Robinson M.M., 2014. Differential effect of endurance training on mitochondrial protein damage, degradation, and acetylation in the context of aging. The Journals of Gerontology Series. 70(11). 359-365.


13.  Kang T., 2013. Exercise training attenuates aging-associated mitochondrial dysfunction in rat skeletal muscle: role of PGC-1alpha. Experimental Gerontology,78(48): 1343-1350.


14.  Khosravan S.H., Alaviani M., Alami A., Tavakolizadeh J., 2014. Epidemiology of loneliness in elderly women. Journal of Research and Health., 80(4): 871-733.


15.  Lanza I.R., Nair K.S., 2009. Muscle mitochondrial changes with aging and exercise. The American Journal of Clinical Nutrition. 89(1): 467S-71S.


16.  Martin J., MacInnis T., Gibala K., 2017 Physiological adaptations to interval training and the role of exercise intensity. Journal of Physiology, 100(9) 2915-2930.


17.  Masataka S., Hiroshi N., Zsolt R., Shuzo K., 2008. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor γ coactivator-1α protein expressions in rat skeletal muscle.  Metabolism Clinical and Experimental Journal, 112(56): 986–998.


18.  Morris B.J., 2013. Seven sirtuins for seven deadly diseases of aging. Free Radical Biology and Medicine, 120(56): 133-171.


19.  O’Hagan K.A., Cocchiglia S., Zhdanov A.V., Tambawala M., Cummins E.P., Monfared M., Agbor T.A., Garvey J.F., Papkovsky D.B., Taylor C.T., Allan B.B., 2009. PGC-1{alpha} is coupled to HIF-1{alpha}-dependent gene expression by increasing mitochondrial oxygen consumption in skeletal muscle cells. Proceedings of the National Academy of Sciences of the United States of America22(13): 755-761.


20.  Ramis R., Esteban S., Miralles A., Reiter R.J., 2015. Caloric restriction, resveratrol and melatonin: Role of SIRT1 and implications for aging andrelated-diseases.  Mechanisms of Ageing and Development, 146(4): 28-41.


21.  Sahin E., Colla S., Liesa M., Moslehi J., Muller F.L., 2011. Telomere dysfunction induces metabolic and mitochondrial compromise. Nature, 470(7334): 359-365.


22.  Seldeen K.L., Lasky G., Leiker M.M., Pang M., Personius K.E., Troen B.R., 2017. High Intensity Interval Training (HIIT) improves physical performance and frailty in aged mice. The Journals of Gerontology, 36 (16): 112-120.


23.  Sharafi D., Soori R., Rastegar Mansouri., 2017. The Effect of High Intensity Interval Training on Muscular Biomarkers of Mitochondrial Biogenesis in Male Rats. Journal of Babol University of Medical Sciences, 120(9): 57-63 (In Persian).


24.  Thomas R., Robergs R.A., Pascoe D., Lambert C., 1991. Adaptations to swimming training: influence of training volume. Medicine and Science in Sports and Exercise, 78(23): 371-377.


25.  Ungvari Z., Sonntag W.E., Csiszar A., 2010. Mitochondria and aging in the vascular system. Journal of Molecular Medicine, 25(5): 1021-1027.


26.  Yujia Y., Philip N., Younan L., 2016. Regulation of SIRT1 in aging: Roles in mitochondrial function and biogenesis. Mechanisms of Ageing and Development.   155(4): 10-21.

26.          Yoho K., Matthew T., 2017. Hood Impact of Aging and Exercise on Mitochondrial Quality Control in Skeletal Muscle. Oxidative Medicine and Cellular Longevity, 66(5): 21-27. 

 Zoltan B., Zhongfu Z., Erika I., 2012. The effects of aging, physical training, and a single bout of exercise on mitochondrial protein expression in human skeletal muscle. Experimental Gerontology, 47(6): 417-424.