The Effect of Different Levels of Dietary Manganese on Antioxidant Activity, Liver Enzymes and Liver Histology in Reared Young Beluga (Huso huso)

Subject Areas : Journal of Animal Biology

Fatemeh Hemmati ¹ , Hossein Khara ² , Habib Vahabzadeh Roudsari ³ , Rezvanollah Kazemi ⁴

1 - Department of Fishery, Lahijan Branch, Islamic Azad University, Lahijan, Iran
2 - Department of Fishery, Lahijan Branch, Islamic Azad University, Lahijan, Iran
3 - Department of Fishery, Lahijan Branch, Islamic Azad University, Lahijan, Iran
4 - Agricultural Research, Education and Extension Organization (AREEO), International Sturgeon Research Institute, Rasht, Iran

Received: 2024-01-16 Accepted : 2024-02-13 Published : 2024-06-01

Keywords: Beluga (Huso huso), dietary manganese, Antioxidant activity, liver enzymes, Liver tissue,

Abstract :

This research aims to determine the effect of different amounts of dietary manganese on antioxidant activity, liver enzymes and, liver tissue of rearing young beluga (Huso huso) from October to December 2022 at the Dr. Beheshti Reproduction and Genetic Stock Restoration Center of Sturgeon in Rasht, Guilan province. For this study, 180 pieces of beluga with an average initial weight of 266 ± 3.05 grams underwent a two-week adaptation period in the breeding environment, in six treatment groups and each treatment with three repetitions, with concentrations of 5 (Mn1), 10 (Mn2), 15 (Mn3), 20 (Mn4) and 25 (Mn5) mg of manganese sulfate monohydrate (MnSO4H2O) per kilogram of food and control treatment (Mn0) without adding manganese sulfate supplement were carried out in two months. At the end of each month, three pieces of fish were selected from each repetition, blood was collected and their livers were sampled for histological studies. The results revealed a significant difference in catalase and glutathione peroxidase levels among the experimental treatments (p<0.05) and their maximum amount was the control treatment fish, while superoxide dismutase levels did not differ significantly (p<0.05). Among the liver enzymes, Alkaline-phosphatase and aspartate-aminotransferase had a significant difference between the control treatment and other experimental treatments (p<0.05), but the alanine-aminotransferase enzyme had no significant difference (p<0.05). Also, different forms of tissue damage were observed in the liver tissue of all treatments, even the control (atrophy, biliary stagnation, Fat degeneration and, cellular necrosis). Based on the results of this research, the levels of 10-15 mg of dietary manganese could improve antioxidant activities, liver enzymes and reduce liver tissue damage in breeding young beluga.

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