Inhibition of Breast Cancer Cells by Microvesicles Containing Doxorubicin

Subject Areas : Journal of Animal Biology

Fatemeh Akhavan Attar ¹ , Shiva Irani ² , mana oloomi ³ , Azam Bolhasani ⁴ , Loabat Geranpayeh ⁵ , Fatemeh Atyabi ⁶

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
4 - Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
5 - Department of 4th Surgery, Sina Hospital, Tehran, Iran
6 - Department of Pharmaceutical Nanotechnology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Received: 2023-06-24 Accepted : 2023-10-15 Published : 2024-06-01

Keywords: Breast cancer, Mesenchymal stem cells, Microvesicle, Doxorubicin,

Abstract :

Breast cancer (BC) is the leading cause of cancer-related death among women in the whole world. Therefore, it is necessary to identify new methods for BC treatment. The purpose of this research is to investigate the effect of microvesicles containing doxorubicin (EV-Dox) on the MCF-7 cell line. Mesenchymal stem cells (MSCs) were isolated from human adipose tissue. Microvesicles secreted from MSCs were extracted by ultracentrifugation. The shape and size of microvesicles were assessed by SEM and DLS, respectively. Doxorubicin (Dox) was loaded into microvesicles by sonication method. MCF-7 cells were treated with different concentrations of Dox (2.5, 5, 10, 20, and 40 µM) for 24 and 48 h and EV-Dox (0.625, 1.25, 2.5, 5, and 10 µM) for 48 and 72h. IC50 was determined based on MTT assay. The findings from SEM and DLS showed that the extracted microvesicles are spherical in shape, with a size of about 592.3 nm. MTT results showed that microvesicles aloan had no significant inhibitory effect on MCF-7 cells, and IC50 of Dox and EV-Dox were reported as 2.2 µM and 2.4 µM, respectively. EV-Dox can be a suitable strategy for treating BC because this study showed that the amount of cell death caused by this type of treatment is higher than the treatment with Dox. Also, the survival assay showed that the amount of apoptosis caused by EV-Dox (56.5%) was significantly higher than Dox (43%) ( p < 0.01).

 

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