The Effect of Six Weeks of Intense Intermittent Exercise and Curcumin on Glucose Levels and BMP-2 Gene Expression in Vascular Smooth Muscle of Diabetic Male Rats
Subject Areas : Journal of Animal Biology
Reza
Sheikh
1
(Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.)
Mohammad
Galehdari
2
(Department of Exercise Physiology, Faculty of Humanities, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.)
Keywords: Interval training, BMP2, Curcumin, Diabetes,
Abstract :
The present study was conducted with the aim of investigating the effect of six weeks of intermittent exercise and curcumin consumption on BMP2 gene expression in the vascular smooth muscle of male Wistar rats. In an experimental trial, 30 male Wistar rats were randomly divided into six groups (5 rats in each group): control, diabetic, healthy intermittent exercise, intermittent exercise diabetes, curcumin diabetes and curcumin exercise diabetes. Diabetes was induced by intraperitoneal injection of streptozotocin poison (50 mg/kg). Intermittent training consisted of 5-12 bouts of intense work (75 to 100% of maximum speed) of 60 seconds with active rest intervals of 75 seconds, six days a week for six weeks. Curcumin was fed to animals by gavage at a dose of 50 mg per kilogram of body weight. After six weeks, unconscious animals and blood samples were collected from their hearts. The data were analyzed using ANOVA statistical test and Benferroni post hoc test. Induction of diabetes increased BMP2 gene expression in vascular smooth muscle of male rats. Intermittent training decreased BMP2 gene expression in vascular smooth muscle of diabetic rats. Six weeks of curcumin consumption reduced the expression of BMP2 gene in vascular smooth muscle of diabetic rats. In general, the results of the present study showed that intermittent exercise and curcumin improve diabetes disorders caused by BMP2 gene expression and calcification and its complications.
1. Adams V., Erbs S. 2014. Influence of exercise training on HDL function. Clinical Lipidology, 9(4):395-398.
2. Ahmadi S., Sheikh R. 2023. Effect of incremental interval training on levels of Fasl protein in lung tissue of mature male Wistar rats: Does exercise training reduce lung inflammation? Journal of Exercise and Organ Cross Talk, 3(2):66-72.
3. Association A.D. 2020. 2. Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes-2020. Diabetes Care, 43(Suppl 1):S14.
4. Basic-Jukic N., Gulin M., Hudolin T., Kastelan Z., Katalinic L., Coric M., Jelakovic B. 2016. Expression of BMP-2 in vascular endothelial cells of recipient may predict delayed graft function after renal transplantation. Kidney and Blood Pressure Research, 41(6):781-793.
5. Bostani M., Sheikh R. 2023. The effect of different degrees of dehydration on the plasma levels of creatine kinase and lactate dehydrogenase of professional wrestlers in Ahvaz. Sports Science Quarterly, 15(49): 49-70.
6. Chen N.X., Duan D., O'Neill K.D., Moe S.M. 2006. High glucose increases the expression of Cbfa1 and BMP-2 and enhances the calcification of vascular smooth muscle cells. Nephrology Dialysis Transplantation, 21(12):3435-3442.
7. Chuengsamarn S., Rattanamongkolgul S., Luechapudiporn R., Phisalaphong C., Jirawatnotai S. 2012. Curcumin extract for prevention of type 2 diabetes. Diabetes Care, 35(11):2121-2127.
8. Fukui N., Zhu Y., Maloney W.J., Clohisy J., Sandell L.J. 2003. Stimulation of BMP-2 expression by pro-inflammatory cytokines IL-1 and TNF-α in normal and osteoarthritic chondrocytes. JBJS, 85(suppl3):59-66.
9. Hruska K.A., Mathew S., Saab G. 2005. Bone morphogenetic proteins in vascular calcification. Circulation research, 97(2):105-114.
10. Iijima H., Ito A., Nagai M., Tajino J., Yamaguchi S., Kiyan W., Aoyama T. 2017. Physiological exercise loading suppresses post-traumatic osteoarthritis progression via an increase in bone morphogenetic proteins expression in an experimental rat knee model. Osteoarthritis and Cartilage, 25(6):964-975.
11. Johnson M.L., Harnish K., Nusse R., Van Hul W. 2004. LRP5 and Wnt signaling: a union made for bone. Journal of Bone and Mineral Research, 19(11):1749-1757.
12. Kurita T., Makino Y. 2013. Novel curcumin oral delivery systems. Anticancer Res, 33(7):2807-2821.
13. Li M., Wu P., Shao J., Ke Z., Li D., Wu J. 2016. Losartan inhibits vascular calcification by suppressing the BMP2 and Runx2 expression in rats in vivo. Cardiovascular Toxicology, 16(2):172-181.
14. Lingli Z., Jianmin G., Jun Z. 2016. Exercise and Mechanical Load Influence the Bone Metabolism through Bone Morphogenetic Protein. Chinese Journal of Cell Biology, 4:16.
15. Little J.P., Safdar A., Wilkin G.P., Tarnopolsky M.A., Gibala M.J. 2010. A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. The Journal of Physiology, 588(Pt 6):1011-1022.
16. Nguyen-Yamamoto L., Tanaka K.I., St–Arnaud R., Goltzman D. 2019. Vitamin D–regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification. JCI Insight, 4(13):e126467.
17. Nomura S., Takano-Yamamoto T. 2000. Molecular events caused by mechanical stress in bone. Matrix Biology, 19(2):91-96.
18. Park J.k., Jang H., Hwang S., Kim E.-J., Kim D.E., Oh K.B., Inoue H. 2014. ER stress-inducible ATF3 suppresses BMP2-induced ALP expression and activation in MC3T3-E1 cells. Biochemical and Biophysical Research Communications, 443(1):333-338.
19. Pulido-Moran M., Moreno-Fernandez J., Ramirez-Tortosa C., Ramirez-Tortosa M. 2016. Curcumin and health. Molecules, 21(3):264.
20. Ravindran J., Prasad S., Aggarwal B.B. 2009. Curcumin and cancer cells: how many ways can curry kill tumor cells selectively? The AAPS Journal, 11(3):495-510.
21. Sage A., Tintut Y., Garfinkel A., Demer L. 2009. Systems biology of vascular calcification. Trends in Cardiovascular Medicine, 19(4):118-123.
22. Sato A.Y.S., Bub G.L., Campos A.H. 2014. BMP-2 and-4 produced by vascular smooth muscle cells from atherosclerotic lesions induce monocyte chemotaxis through direct BMPRII activation. Atherosclerosis, 235(1):45-55.
23. Scalbert A., Manach C., Morand C., Rémésy C., Jiménez L. 2005. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition, 45(4):287-306.
24. Sheikh R., Gallehdari M. 2023. The effect of herbal supplement and exercise training on plasma lipid profile in diabetic male rats. Journal of Exercise and Organ Cross Talk, 3(2):86-92.
25. Sheikh R., Habibi A. 2023. Exercise training increases the chance of the body's immune system to fight against the disease of Covid-19: A mini review of exercise, immune system and myokines. Journal of Exercise and Organ Cross Talk, 3(3):150-155.
26. Sheikh R., Nikbakht M. 2023. Physical activity and apoptosis, a brief review of previous findings. doi: 10.22059/sshr.2023.360921.1097
27. Sheikh R., Shakeryan S., Tabatbaei S. R.F., Habibi A. 2023. Moderate and high-intensity interval training protect against diabetes-induced modulation of hepatic CD86 and CD206 expression associated with the amelioration of insulin resistance and inflammation in rats. Immunobiology, 228(6):152745.
28. Singh S. 2007. From exotic spice to modern drug? Cell, 130(5):765-768.
29. Soleimani N., Gallehdari M., Sheikh R. 2023. Effect of six weeks of interval training and curcumin consumption on apolipoprotein A and B in diabetic male rats. Journal of Exercise and Organ Cross Talk, 3(2):73-80.
30. Son H.E., Kim E.J., Jang W.G. 2018. Curcumin induces osteoblast differentiation through mild-endoplasmic reticulum stress-mediated such as BMP2 on osteoblast cells. Life Sciences, 193:34-39.
31. Su X., Ao L., Shi Y., Johnson T.R., Fullerton D.A., Meng X. 2011. Oxidized low density lipoprotein induces bone morphogenetic protein-2 in coronary artery endothelial cells via Toll-like receptors 2 and 4. Journal of Biological Chemistry, 286(14):12213-12220.
32. Sun M., Chang Q., Xin M., Wang Q., Li H., Qian J. 2017. Endogenous bone morphogenetic protein 2 plays a role in vascular smooth muscle cell calcification induced by interleukin 6 in vitro. International Journal of Immunopathology and Pharmacology, 30(3):227-237.
33. Sun Z., Wan G., Liang S., Qian C. 2019. Effect of bone morphogenetic protein-2 on diabetic retinopathy and its mechanism of action. Tropical Journal of Pharmaceutical Research, 18(5): 1069-1076.
34. Trzeciakiewicz A., Habauzit V., Horcajada M.N. 2009. When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols. Nutrition Research Reviews, 22(1):68-81.
35. Urist M.R. 1965. Bone: formation by autoinduction. Science, 150(3698):893-899.
36. Van Der Horst G., van der Werf S.M., Farih‐Sips H., van Bezooijen R.L., Löwik C.W., Karperien M. 2005. Downregulation of Wnt signaling by increased expression of Dickkopf‐1 and‐2 is a prerequisite for late‐stage osteoblast differentiation of KS483 cells. Journal of Bone and Mineral Research, 20(10):1867-1877.
37. Wang L., Zhang B., Huang F., Liu B., Xie, Y. 2016. Curcumin inhibits lipolysis via suppression of ER stress in adipose tissue and prevents hepatic insulin resistance. Journal of Lipid Research, 57(7):1243-1255.
38. Westendorf J.J., Kahler R.A., Schroeder T.M. 2004. Wnt signaling in osteoblasts and bone diseases. Gene, 341: 19-39.
39. Yao Y., Bennett B.J., Wang X., Rosenfeld M.E., Giachelli C., Lusis A.J., Boström K.I. 2010. Inhibition of bone morphogenetic proteins protects against atherosclerosis and vascular calcification. Circulation Research, 107(4):485-494.
40. Zhang L., Sun H., Liu S., Gao J., Xia J. 2019. Glycemic variability is associated with vascular calcification by the markers of endoplasmic reticulum stress-related apoptosis, Wnt1, galectin-3 and BMP-2. Diabetology and Metabolic Syndrome, 11(1):67.
